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首页> 外文期刊>International Journal of Neonatal Screening >A Simple Method to Overcome the ?￠????Floating Disc Problem?￠???? Using the GALT-Assay on the PerkinElmer GSP?￠????Remeasurement on a Stand Alone Plate Fluorimeter
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A Simple Method to Overcome the ?￠????Floating Disc Problem?￠???? Using the GALT-Assay on the PerkinElmer GSP?￠????Remeasurement on a Stand Alone Plate Fluorimeter

机译：一种解决？￠ ??????浮盘问题？Simple ????的简单方法在PerkinElmer GSP上使用GALT分析-在独立平板荧光计上进行重新测量

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The Perkin Elmer Genetic Screening Processor (GSP)?￠???￠ is a fully automated system for the processing of immunoassays for thyroid stimulating hormone (TSH), 17-hydroxyprogesterone (17-OHP), immuno reactive trypsin (IRT), biotinidase, and total T4, as well as enzymatic assays for total galactose and galactose-1-phosphate uridyltransferase (GALT) from dried blood spots (DBS). The system however, has one drawback: it cannot transfer samples from one microtiter plate to another. While this is not a problem for immunoassays, it makes enzymatic assays more problematic. The remaining DBS can either cause significant signal quenching, or they can increase fluorescence intensity, when the DBS are floating on the surface. The latter can cause false negative results, when GALT is measured for galactosaemia screening. To overcome this problem, an additional measurement step to check for floating disks is incorporated, leading to prevention of the affected measurements. However, this causes a secondary problem in this totally closed system. We detected floating disk signals in approx. 0.7% of all screening samples as well as quality control samples, which had to be repeated. We describe a simple method, which is just a re-measurement on a victor fluorescence reader, or any other plate fluorimeter, with filters for excitation wavelength 340 nm, and emission wavelength 405 nm. The introduction of this second-tier measurement made all repeat measurements unnecessary.
机译：珀金埃尔默遗传筛选处理器（GSP）是用于处理甲状腺刺激激素（TSH），17-羟基孕酮（17-OHP），免疫反应性胰蛋白酶（IRT），生物素酶的免疫测定的全自动系统，总T4，以及酶法测定干血斑（DBS）中的总半乳糖和半乳糖1-磷酸尿嘧啶转移酶（GALT）。然而，该系统具有一个缺点：它不能将样品从一个微量滴定板转移到另一个。尽管这对于免疫测定法不是问题，但它使酶法测定法更具问题。当DBS漂浮在表面上时，剩余的DBS可能导致明显的信号猝灭，或者它们可以增加荧光强度。当对GALT进行半乳糖血症筛查时，后者可能导致假阴性结果。为了克服这个问题，增加了一个额外的测量步骤来检查浮动磁盘，从而防止了受影响的测量。但是，这在这个完全封闭的系统中引起了第二个问题。我们大约检测到浮动磁盘信号。所有筛选样品以及质量控制样品中的0.7％，必须重复进行。我们描述了一种简单的方法，该方法只是在victor荧光读取器或任何其他平板荧光计上进行的重新测量，带有用于激发波长340 nm和发射波长405 nm的滤光片。第二层测量的引入使所有重复测量都不再需要。

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