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首页> 外文期刊>International Journal of Plant Genomics >Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop
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Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop

机译：在生物量作物大草原草中实现标记辅助选择育种计划的进展

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Prairie cordgrass (Spartina pectinataBosc ex Link) is an indigenous, perennial grass of North America that is being developed into a cellulosic biomass crop suitable for biofuel production. Limited research has been performed into the breeding of prairie cordgrass; this research details an initial investigation into the development of a breeding program for this species. Genomic libraries enriched for four simple sequence repeat (SSR) motifs were developed, 25 clones from each library were sequenced, identifying 70 SSR regions, and primers were developed for these regions, 35 of which were amplified under standard PCR conditions. These SSR markers were used to validate the crossing methodology of prairie cordgrass and it was found that crosses between two plants occurred without the need for emasculation. The successful cross between two clones of prairie cordgrass indicates that this species is not self-incompatible. The results from this research will be used to instigate the production of a molecular map of prairie cordgrass which can be used to incorporate marker-assisted selection (MAS) protocols into a breeding program to improve this species for cellulosic biomass production.
机译：草原草（Spartina pectinataBosc ex Link）是北美的一种多年生原生草，正在被开发为适合生物燃料生产的纤维素生物质作物。草原草的育种研究有限。这项研究详细介绍了对该物种育种计划的发展的初步调查。开发了富集了四个简单序列重复（SSR）基序的基因组文库，对每个文库的25个克隆进行了测序，确定了70个SSR区域，并开发了这些区域的引物，其中35个在标准PCR条件下扩增。这些SSR标记用于验证草原草的杂交方法，发现两株植物之间的杂交不需要去雄。草原草两个克隆之间的成功杂交表明该物种不是自我不相容的。这项研究的结果将用于促进草原草皮分子图谱的产生，该图谱可用于将标记辅助选择（MAS）方案纳入育种程序中，以改善该物种的纤维素生物质生产能力。

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