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首页> 外文期刊>International Journal of Phytopharmacy >Pharmacognostical Standardization, Phytochemical Analysis and In-vitro Antioxidant Activity of rhizome of Polygonum alpinum All.
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Pharmacognostical Standardization, Phytochemical Analysis and In-vitro Antioxidant Activity of rhizome of Polygonum alpinum All.

机译：何首乌的根茎的药理学标准化，植物化学分析和体外抗氧化活性

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摘要

The present study was designed to evaluate the pharmacognostic characters, physicochemical parameters and in-vitro antioxidant activity of rhizome of Polygonum alpinum. The powder microscopy revealed the presence of epidermis, hypodermis with calcium oxalate crystals, non lignified vascular bundle, calcium oxalate crystals, cork in surface view and fragments of bordered pitted vessels. Phytochemical screening of various extracts revealed the presence of carbohydrates, cardiac glycosides, coumarin, steroids, terpenoids, flavonoids, saponins and tannins. Physiochemical analysis showed 2.325% of total ash, 0.525% of water soluble ash, 0.650% of acid insoluble ash and 3.625% of sulphated ash w/w. Other parameters like extractive value, foreign matter, moisture content, swelling index, foaming index, pH of different solvents, total tannin content, total fat content and fluorescence analysis were also determined. The total phenolics in alcoholic and aqueous extract was found to be 41.06 and 23.30 mg/g GAE respectively while that of flavonoid content in alcoholic and aqueous extract was 182.13 and 123.71 mg/g RE respectively. The percentage inhibition of DPPH by alcoholic and aqueous extract at a concentration of 100 μg/mL was found to be 96.50% and 49.44% respectively. The reducing power of alcoholic and aqueous extract at 100 μg/mL was found to be 0.295 and 0.175 respectively and increased to 0.765 and 0.596 respectively at 500 μg/mL. The results indicate that alcoholic extract has potent antioxidant activity as compared to the aqueous extract.
机译：本研究旨在评估何首乌的根茎的药理学特征，理化参数和体外抗氧化活性。粉末显微镜检查发现存在表皮，皮草和草酸钙晶体，未木质化的血管束，草酸钙晶体，软木塞和表面有斑点的血管碎片。对各种提取物进行植物化学筛选后发现存在碳水化合物，强心苷，香豆素，类固醇，萜类，黄酮类，皂苷和单宁酸。理化分析显示总灰分的2.325％，水溶性灰分的0.525％，酸不溶性灰分的0.650％和硫酸灰分的w / w的3.625％。还确定了其他参数，如萃取值，异物，水分含量，溶胀指数，发泡指数，不同溶剂的pH，单宁含量，总脂肪含量和荧光分析。酒精和水提取物中的总酚含量分别为41.06和23.30 mg / g GAE，而酒精和水提取物中的类黄酮含量分别为182.13和123.71 mg / g RE。浓度为100μg/ mL的乙醇提取物和水提取物对DPPH的抑制百分比分别为96.50％和49.44％。酒精和水提取物在100μg/ mL时的还原力分别为0.295和0.175，在500μg/ mL时分别降低至0.765和0.596。结果表明，与水提取物相比，酒精提取物具有有效的抗氧化活性。

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