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首页> 外文期刊>International Journal of Phytopharmacy >EVALUATION OF ANTI ARTHRITIC ACTIVITY OF THE ROOT EXTRACT OF ACALYPHA INDICA LINN. USING IN VITRO TECHNIQUES
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EVALUATION OF ANTI ARTHRITIC ACTIVITY OF THE ROOT EXTRACT OF ACALYPHA INDICA LINN. USING IN VITRO TECHNIQUES

机译：刺槐根提取物抗关节炎活性的评价。体外技术的使用

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Acalypha indica L., belonging to family Euphorbiaceae, is an herb used in traditional medicine for the?treatment of bronchitis, asthma, pneumonia, rheumatism, earache, syphilitic ulcers, bacterial and renal?diseases. Acalypha indica crude root powder was extracted with solvents of increasing polarity viz.?petroleum ether, chloroform and methanol successively by continuous hot percolation method. Acalypha indica methanol extract was evaluated using three different in vitro models to explore antiarthritic potential such as inhibition of protein denaturation, proteinase inhibitory action and antihyaluronidase activity. The concentrations of 10 to 200 μg/ml of Acalypha indica methanol extract?were prepared using DMSO. Diclofenac was used as the positive control. All in vitro determinations?were done in triplicate. A dose dependent increase in percentage inhibition was observed for all the three models. The inhibitory concentration (IC50) was found to be 52 μg/ml for protein denaturation?assay, 37 μg/ml in proteinase inhibitory action and 18 μg/ml for anti-hyaluronidase activity.?Diclofenac offered protective activity at even much lower concentrations compared to Acalypha indica methanol extract producing IC50 values of 40 and 13 μg/ml for protein denaturation and?proteinase inhibitory assays. Acalypha indica exhibited a very good anti-arthritic activity in all the?methods checked confirming its traditional use. Further in vivo studies are to be carried out to confirm their activity and explore the mechanism by which this plant acts in protecting from?autoimmune disease and rationalize their use.
机译：属于印度大戟科的印度ca虫（Acalypha indica L.）是一种传统药物，用于治疗支气管炎，哮喘，肺炎，风湿病，耳痛，梅毒性溃疡，细菌和肾脏疾病。通过连续热渗滤法依次用极性递增的溶剂（如石油醚，氯仿和甲醇）提取树粗制根粉。使用三种不同的体外模型评估了印度ca桐的甲醇提取物，以探索抗关节炎的潜力，例如抑制蛋白质变性，抑制蛋白酶的作用和抗透明质酸酶的活性。使用DMSO制备浓度为10到200μg/ ml的印度ca树甲醇提取物。双氯芬酸用作阳性对照。所有体外测定均重复三次。对于所有三个模型，观察到剂量抑制百分比的依赖性增加。蛋白质变性检测的抑制浓度（IC50）为52μg/ ml，蛋白酶抑制作用的抑制浓度为37μg/ ml，抗透明质酸酶活性的检测浓度为18μg/ ml。双氯芬酸在更低的浓度下提供了保护活性。用于蛋白质变性和蛋白酶抑制分析的印度A叶甲醇提取物的IC50值分别为40和13μg/ ml。印加沙棘在所有检查方法中均表现出很好的抗关节炎活性，证实了其传统用途。将进行进一步的体内研究以确认其活性并探索该植物在预防自身免疫性疾病和合理化其使用中起作用的机制。

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