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首页> 外文期刊>International Journal of Phytopharmacy >Quantitative Estimation of Andrographolide by Reverse Phase-High Liquid Chromatography Method from Andrographis Paniculata Nees.
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Quantitative Estimation of Andrographolide by Reverse Phase-High Liquid Chromatography Method from Andrographis Paniculata Nees.

机译：穿心莲中反相高效液相色谱法定量测定穿心莲内酯的含量。

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摘要

Reverse Phase High performance liquid chromatographic method with UV array detection was established for the determination of Andrographolide. The Andrographolide was separated using isocratic solvent system consisting of isopropyl alcohol, formic acid and water (70:10:20 v/v) at flow rate of 1.0 ml/min and the detection wavelength of 223 nm. The method was validated for linearity, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ). The linearity of the proposed method was obtained in the range of 4.5-70 μg/ml for andrographolide with regression coefficient of 0.9999. Intraday and interday precision studies showed the relative standard deviation less than 2.5%. The accuracy of the proposed method was determined by a recovery study conducted at 3 different levels. The average recovery was 97-99%. The LOD and LOQ were 0.03 and 0.05 μg/ml for andrographolide, respectively. The contents of andrographolide obtained in the dried leaves powder was within the ranges of 0.98 – 1.15% w/w, respectively. The proposed method is simple, sensitive yet reproducible. It is therefore suitable for routine analysis of andrographolide in A. Paniculata Nees.
机译：建立了采用紫外阵列检测的反相高效液相色谱法测定穿心莲内酯的含量。使用由异丙醇，甲酸和水（70:10:20 v / v）组成的等度溶剂系统以1.0 ml / min的流速和223 nm的检测波长分离穿心莲内酯。验证了该方法的线性，精密度，准确性，检测限（LOD）和定量限（LOQ）。穿心莲内酯的线性度在4.5-70μg/ ml范围内，回归系数为0.9999。日内和日间精度研究表明相对标准偏差小于2.5％。通过在3个不同级别进行的回收率研究确定了所提出方法的准确性。平均回收率为97-99％。穿心莲内酯的LOD和LOQ分别为0.03和0.05μg/ ml。干叶粉中穿心莲内酯的含量分别在0.98 – 1.15％w / w的范围内。所提出的方法简单，灵敏但可重现。因此，它适合常规分析A. Paniculata Nees中的穿心莲内酯。

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