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首页> 外文期刊>International Journal of Poultry Sciences >Haplotype Structure and DNA Sequence Variation of the Liver Expressed Antimicrobial Peptide-2 ( chLEAP-2 ) Gene in Chickens challenged with Eimeria maxima
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Haplotype Structure and DNA Sequence Variation of the Liver Expressed Antimicrobial Peptide-2 ( chLEAP-2 ) Gene in Chickens challenged with Eimeria maxima

机译：巨大艾美球虫攻击的鸡肝脏表达的抗菌肽-2（chLEAP-2）基因的单倍型结构和DNA序列变异

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Objective: The aim of this study was to screen the chLEAP-2 gene for DNA sequence variation and to evaluate the relationships among its haplotypes (based on haplogroups), expression levels, weight gain and lesion score in two chicken lines challenged with Eimeria maxima . Methodology: A total DNA sequence of 4.6 kb including the chLEAP-2 gene was screened by re-sequencing of individual amplicons. Sixteen SNPs, including seven each in the promoter and introns and two in exons, were identified. Results: One of the exonic single-nucleotide polymorphism (SNPs) was non-synonymous, involving a cysteine to tyrosine codon change. About 25% of the SNPs were in Hardy Weinberg equilibrium. Linkage disequilibrium (D?) among the SNPs ranged from 0.02-1.00. The haplotypes observed from the 16 SNPs were assembled into 5 haplogroups. The estimated frequencies of the haplogroups ranged from 0.17-0.23 in the combined chicken lines. Although not significant (p > 0.05), the chLEAP-2 gene expression varied among haplogroups. Differences among haplogroups for lesion score and weight gain were consistent, but not statistically significant (p > 0.05). However, Hap4 appeared to be the haplogroup least susceptible to coccidiosis. At a minimum, the data do not support an association between chLEAP-2 DNA sequence variation and symptoms of coccidiosis such as weight gain depression and lesion score. Conclusion: Therefore, earlier reports of differences between resistant and susceptible lines in chLEAP-2 expression may be due to trans-acting factors. The genomic results reported here provide resources for testing the trans-expression control theory and will be useful for future genotype:phenotype evaluation studies between chLEAP-2 and other traits in the chicken.
机译：目的：本研究的目的是筛选chLEAP-2基因的DNA序列变异，并评估在两个最大艾美球虫攻击的鸡系中其单倍型（基于单倍群），表达水平，体重增加和病变得分之间的关​​系。方法：通过对单个扩增子进行重新测序，筛选出包含chLEAP-2基因的4.6 kb总DNA序列。确定了16个SNP，其中每个在启动子和内含子中七个，在外显子中两个。结果：外显子单核苷酸多态性（SNPs）之一是非同义词，涉及半胱氨酸到酪氨酸密码子的变化。大约25％的SNP处于Hardy Weinberg平衡状态。 SNP之间的连锁不平衡（D 2）为0.02-1.00。从16个SNP中观察到的单倍型被组装成5个单倍型。在组合鸡品系中，单倍群的估计频率为0.17-0.23。尽管不显着（p> 0.05），但chLEAP-2基因表达在单倍组之间有所不同。单倍组之间的病变评分和体重增加的差异是一致的，但无统计学意义（p> 0.05）。但是，Hap4似乎是球虫病最不易感染的单倍群。至少，该数据不支持chLEAP-2 DNA序列变异与球虫病症状（如体重增加抑郁和病灶评分）之间的关联。结论：因此，有关chLEAP-2表达中抗性和易感品系之间差异的早期报道可能是由于反式作用因子引起的。此处报道的基因组结果为测试反式表达控制理论提供了资源，并将对未来chLEAP-2与鸡其他性状之间的基因型：表型评估研究有用。

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