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首页> 外文期刊>International Journal of Poultry Sciences >Association of ApoVLDLII Gene Polymorphism with Body Composition Traits in Kampung Chicken
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Association of ApoVLDLII Gene Polymorphism with Body Composition Traits in Kampung Chicken

机译：甘榜鸡ApoVLDLII基因多态性与体成分性状的相关性

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Background and Objective: Very low density lipoproteins (VLDLs) is a major class of lipoprotein particles that is synthesized and secreted by the liver. Selection for economic traits based on molecular marker assisted selection are required to increase production performance. The present study was designed to analyze associations of very low density Apo lipoprotein II (ApoVLDLII) gene polymorphisms with body composition traits. Kampung chicken, a native chicken in Indonesia, is slow-growing chicken. Materials and Methods: A total of 76 male Kampung chickens were used in the current study. Body composition s were measured in 12 and 26 weeks of age. Primers for intron 1 region were designed from genomic chicken sequence. A G634A SNP of the ApoVLDLII gene intron 1 region was detected and polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) method was then used for genotyping of Kampung chicken population. Results: The ApoVLDLII polymorphism was significantly associated with body, carcass, breast, thigh, back and thigh muscle weight in 26 weeks old Kampung chicken population (pConclusion: It is concluded that the ApoVLDLII gene could be a candidate gene that affects growth and body composition traits in chicken.
机译：背景与目的：超低密度脂蛋白（VLDLs）是由肝脏合成和分泌的一类主要脂蛋白颗粒。需要基于分子标记辅助选择的经济性状选择，以提高生产性能。本研究旨在分析极低密度Apo脂蛋白II（ApoVLDLII）基因多态性与身体成分特征的关联。 Kampung鸡是印度尼西亚的本地鸡，是生长缓慢的鸡。材料与方法：本研究共使用76只雄性甘榜鸡。在12和26周龄时测量身体成分。内含子1区的引物是根据基因组鸡序列设计的。检测到ApoVLDLII基因内含子1区域的G634A SNP，然后使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对甘榜鸡群进行基因分型。结果：在26周大的甘榜鸡群中，ApoVLDLII多态性与体重、,体，胸部，大腿，背部和大腿肌肉的重量显着相关（结论：ApoVLDLII基因可能是影响生长和身体组成的候选基因）鸡的性状。

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