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首页> 外文期刊>International Journal of Pharmacological Research >Evaluation of the Total antioxidant capacity of the selected plant extracts using Ferric Reducing Antioxidant Power (FRAP) Assay
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Evaluation of the Total antioxidant capacity of the selected plant extracts using Ferric Reducing Antioxidant Power (FRAP) Assay

机译：使用三价铁抗氧化能力（FRAP）分析评估所选植物提取物的总抗氧化能力

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Back ground: Antioxidants are compounds capable to either delay or inhibit the oxidation processes which occur under the influence of atmospheric oxygen or reactive oxygen species. The cumulative and synergistic activities of the bioactive molecules present in plants are responsible for their enhanced antioxidant properties. The present study is aimed at the in vitro evaluation of the Total antioxidant capacity of the plant extracts of Kydia calycina aerial parts, Alysicarpus monilifer whole plant and Aglaia elaeagnoidea stem.Method: The FRAP method relies on the reduction by the antioxidants, of the complex ferric ion-TPTZ (2,4,6-tri(2-pyridyl)- 1,3,5-triazine). The binding of Fe2+ to the ligand creates a very intense navy blue colour. The absorbance can be measured to test the amount of iron reduced and can be correlated with the amount of antioxidants and their total antioxidant capacity..Result: From the results it was found that, among the hydro alcoholic extracts evaluated, Kydia calycina plant extract showed highest FRAP value (1.41) and Alysicarpus monilifer showed lowest value (0.88).Conclusion: The total antioxidant potential is a relevant tool for investigating the relationship between plant derived antioxidants and pathologies induced by the oxidative stress. In the present investigation, comparative evaluation of Total antioxidant activity of various extracts of selected medicinal plants in comparison to Ascorbic acid exhibited significant results in vitro antioxidant assays.
机译：背景：抗氧化剂是能够延迟或抑制在大气氧或活性氧物种的影响下发生的氧化过程的化合物。植物中存在的生物活性分子的累积和协同活性是其增强的抗氧化特性的原因。本研究的目的是体外评估Kydia calycina地上部分，Alysicarpus monilifer整株植物和Aglaia elaeagnoidea茎的植物提取物的总抗氧化能力。方法：FRAP方法依靠复合物的抗氧化剂还原铁离子-TPTZ（2,4,6-三（2-吡啶基）-1,3,5-三嗪）。 Fe 2+与配体的结合产生深蓝色。可以测量吸光度来测试还原铁的含量，并可以将其与抗氧化剂的量及其总抗氧化能力相关。结果：从结果中发现，在评价的水醇提取物中，Kydia calycina植物提取物显示出最高的FRAP值（1.41）和最低的Alysicarpus monilifer（0.88）。在本研究中，与抗坏血酸相比，所选药用植物的各种提取物的总抗氧化剂活性的比较评价显示出显着的体外抗氧化剂测定结果。

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