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首页> 外文期刊>International Journal of Pharmaceutical Sciences Review and Research >Isolation and Screening of Antimicrobial Actinomycetes from the Soil Surrounding Different Medicinal Plants of Saurashtra with future scope to Produce Antimicrobial Compounds therefrom.
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Isolation and Screening of Antimicrobial Actinomycetes from the Soil Surrounding Different Medicinal Plants of Saurashtra with future scope to Produce Antimicrobial Compounds therefrom.

机译：从Saurashtra的不同药用植物周围土壤中分离和筛选抗菌放线菌，具有从中生产抗菌化合物的未来范围。

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The need for new and useful compounds to provide assistance and relief in all aspects of the human condition is ever growing. The screening approach has been employed extensively in the search for microorganisms capable of producing useful antibiotics. Actinomycetes have the capability to synthesize those substances. The object behind this research is isolation and screening of Actinomycetes producing new useful antimicrobial compound which may cause death of the microbes which has been got resistance. In our study we are selected 11 medicinal plants from 3 regions of saurashtra. Soil sample collected from microflora part up to 20 cm depth of soil, diluted up to 106. Different media used for isolation and make pure colony of actinomycetes. Colonies of actinomycets were selected then color of Aerial and vegetative mycelium was studied. Cultures of isolates were prepared on ISP media & morphological charecteristics studied. Out of 66 isolates 58 isolates were identified up to generic level. For primary screening for antimicrobial activity we used cross streak method. It was found that out of 66 isolates 45 (68.2 %) isolates were showing activity against test microbes and 21 (31.8 %) isolates not showing any activity. It was concluded that actinomycetes isolates from different soil samples around medicinal plant area of saurashtra region are good source to produce antimicrobial compounds. Too many ecological niches still remain unexplored, needs to be studied for novel actinomycetes.
机译：对于新型和有用的化合物以在人类状况的各个方面提供帮助和缓解的需求日益增长。筛选方法已广泛用于寻找能够产生有用抗生素的微生物。放线菌具有合成这些物质的能力。这项研究的目的是分离和筛选放线菌，这些放线菌会产生新的有用的抗菌化合物，这些化合物可能导致已经产生耐药性的微生物死亡。在我们的研究中，我们从saurashtra的3个地区中选择了11种药用植物。从微生物区系中收集的土壤样品的深度可达20厘米，稀释至106厘米。使用不同的培养基进行分离，并形成放线菌的纯菌落。选择放线菌的菌落，然后研究气生菌丝和营养菌丝的颜色。分离物的培养是在ISP培养基和所研究的形态特征上制备的。在66个分离株中，有58个分离株被鉴定到通用水平。对于抗微生物活性的初步筛选，我们使用交叉条纹法。发现在66种分离物中，有45种（68.2％）分离物显示出对测试微生物的活性，而21种（31.8％）分离物没有显示出任何活性。得出的结论是，在索拉什特拉地区药用植物区域周围不同土壤样品中分离到的放线菌是生产抗菌化合物的良好来源。仍然有许多生态位尚未开发，需要研究新型放线菌。

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