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首页> 外文期刊>American Journal of Medicine Studies >Immunogenicity of Leishmania Donavani Centrin-3 Vaccines
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Immunogenicity of Leishmania Donavani Centrin-3 Vaccines

机译：利什曼原虫Donavani Centrin-3疫苗的免疫原性

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Leishmaniasis is a parasitic protozoal disease affecting humans and animals with phlebotomine sand flies as intermediate vectors. There is no effective vaccine in use against this parasite and production relies on finding potent immunogenic antigens with long lasting memory response. As part of searching for new Leishmania antigens of a potential vaccine application, the immunogenicity of L. donovani centrin-3 (Ldcen-3) was investigated in a Balb/c model. Ldcen-3 is a calcium binding protein that has been shown to be involved in duplication and segregation of the centrosome in higher and lower eukaryotes. The Ldcen-3 gene was cloned in various vectors and coated on gold particles for gene gun immunisation. Significant protection was induced by immunisation with 1μg DNA of pcDNA3.1-Ldcen-3 or pCRT7/CT-TOPO-Ldcen-3 constructs. Protection against challenge with live parasite was vector dependent where better protection was induced by pCRT7/CT-TOPO-Ldcen-3. Splenocytes from Balb/c mice immunised with pcDNA3.1-Ldcen-3 or pCRT7/CT-TOPO-Ldcen-3 had a potent CTL response against DC targets loaded with or tumour cells transfected with Ldcen-3 plasmid construct.
机译：利什曼病是一种寄生虫的原生动物疾病，它以人参le蝇为媒介，影响人类和动物。没有针对这种寄生虫的有效疫苗，生产依赖于寻找具有长效记忆反应的有效免疫原性抗原。作为寻找可能的疫苗应用的新利什曼原虫抗原的一部分，在Balb / c模型中研究了多诺威尼森中心蛋白3（Ldcen-3）的免疫原性。 Ldcen-3是一种钙结合蛋白，已被证明与高等和低等真核生物中中心体的复制和分离有关。将Ldcen-3基因克隆到各种载体中，并包被在金颗粒上进行基因枪免疫。通过用1μgDNA的pcDNA3.1-Ldcen-3或pCRT7 / CT-TOPO-Ldcen-3构建体免疫诱导了重要的保护作用。活寄生虫攻击的保护作用依赖于载体，其中pCRT7 / CT-TOPO-Ldcen-3可以诱导更好的保护作用。用pcDNA3.1-Ldcen-3或pCRT7 / CT-TOPO-Ldcen-3免疫的Balb / c小鼠的脾细胞对装载或用Ldcen-3质粒构建体转染的肿瘤细胞的DC靶标具有有效的CTL应答。

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