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首页> 外文期刊>International Journal of Dental Research >Rehydration of air dried oral smears using honey
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Rehydration of air dried oral smears using honey

机译：用蜂蜜对风干的口腔涂片补液

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Background: Papanicolaou stains, very reliable cytological staining in diagnosis of oral carcinoma. In routine practice, 95% ethanol is used to fix the slides while preparing for Pap smears. The problems associated with pap smear such as short of supply and storage of alcohol, wet fixing of slides and transporting them to the cytology centre if the Pap smears are prepared at a peripheral centre. If an alternative method of rehydrating the air-dried oral smears is known we can overcome these problems. Until the date only few studies regarding rehydration of oral smears as a substitute for wet fixation. Aim: The aim of this study is to use honey as an alternative for rehydrating air dried oral smears. Material and methods: This study was done over than 3 months. Two sets of oral smears wet and air dried ,Overall 40 smears were examined; The first set were fixed in 95% ethyl alcohol. The other set of smears were air dried and rehydrated with honey solution(2 parts honey and 1 part saline)Both the smears were stained with Pap stain, examined for preservation of cytomorphological features and evaluated by two experienced pathologists using the light microscope. Results and analysis: This study revealed that immersion of air dried PAP smears in honey for 30 minutes is effectively same as wet smear which is routine method Conclusion: Rehydration, followed by fixation of air-dried smears, is a simple, feasible, applicable and reliable fixation technique, which is comparable to the wet-fixed conventional technique, used for oral smears and can be applied for evaluation on a routine basis.
机译：背景：Papanicolaou染色，诊断口腔癌非常可靠的细胞学染色。在常规实践中，准备进行子宫颈抹片检查时，使用95％的乙醇固定玻片。如果在外周中心准备子宫颈抹片涂片，与子宫颈抹片涂片相关的问题包括：酒精的供应和储存不足，载玻片的湿固定以及将其运送到细胞学中心。如果已知为风干口腔涂片补液的另一种方法，我们可以克服这些问题。迄今为止，只有很少的关于补液替代湿法固定的研究。目的：本研究的目的是使用蜂蜜替代空气干燥的口腔涂片补液。材料和方法：这项研究进行了超过3个月。两组口腔涂片湿润和风干，共检查40涂片;第一组固定在95％的乙醇中。将另一套涂片风干并用蜂蜜溶液（2份蜂蜜和1份盐水）再水化。将这两套涂片均用Pap染色，检查其细胞形态学特征的保留，并由两名经验丰富的病理学家使用光学显微镜进行评估。结果与分析：这项研究表明，风干的PAP涂片在蜂蜜中浸泡30分钟与湿涂片有效，这是常规方法。结论：补液，然后固定风干涂片是一种简单，可行，适用且可操作的方法。可靠的固定技术，与湿法固定的常规技术相当，可用于口腔涂片检查，并可用于常规评估。

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