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Isolation of potential bacterial pathogens from the phylloplane of some selected medicinal plants

机译:从某些精选药用植物的叶状体中分离出潜在的细菌病原体

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Microorganisms are ubiquitous and their impact could be appreciated directly or indirectly. This study was aimed at determining the occurrence of pathogenic bacteria on the phylloplane of some medicinal plants commonly used to treat diseases by oral administration. Leaves samples were collected aseptically from Cassia fistula (Cassia) Mangifera indica (Mango) and Psidium guajavum (Guava) and their bacterial contents were assessed. Higher bacterial counts were observed in raw samples followed by those washed with sterile water. Samples washed with sterile warm water (blanched) were shown to have lower counts although without statistical significance (p ≤ 0.05). Guava leaves had higher counts (3.4×106 cfu/g) in the raw samples and cassia in blanched (6.4×105 cfu/g). However, mango leaves had the lowest (8.4×105 cfu/g and 2.0×105 cfu/g) in both the treatments respectively. Twenty bacterial species were isolated comprising Gram negative and Gram positive species. Bacterial identification revealed that Proteus mirabilis (35%), Proteus vulgaris (15%), Escherichia coli (15%), Klebsiella pneumoniae (5%), Morganella morganii (5%), Salmonella typhi (10%), Enterobacter sp. (5%), Staphylococcus aureus (5%) and Vibrio cholarae (5%) were the prevalent species. These organisms could be potential pathogens and proper washing with hot water may serve a better means of reducing the microbial contents and thus, it is recommended.
机译:微生物无处不在,其影响可以直接或间接地理解。这项研究的目的是确定通常用于口服治疗疾病的一些药用植物的叶平面上病原菌的存在。从决明子瘘(决明子),印度芒果(芒果)和番石榴(番石榴)中无菌采集叶子样品,并评估其细菌含量。在原始样品中观察到更高的细菌计数,然后是用无菌水洗涤的细菌。尽管没有统计学意义(p≤0.05),但用无菌温水(漂白)洗涤的样品显示计数较低。原始样品中番石榴叶的含量更高(3.4×106 cfu / g),而决明子中的决明子含量更高(6.4×105 cfu / g)。然而,在两种处理中,芒果叶分别最低(8.4×105 cfu / g和2.0×105 cfu / g)。分离出二十种细菌,包括革兰氏阴性和革兰氏阳性。细菌鉴定显示,奇异变形杆菌(35%),寻常变形杆菌(15%),大肠杆菌(15%),肺炎克雷伯菌(5%),摩根氏摩根氏菌(5%),伤寒沙门氏菌(10%),肠杆菌。 (5%),金黄色葡萄球菌(5%)和霍乱弧菌(5%)是流行的物种。这些生物可能是潜在的病原体,用热水适当洗涤可能是减少微生物含量的更好方法,因此,建议使用。

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