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首页> 外文期刊>International journal of soil science >Insoluble Phosphate Solubilization by Bacterial Strains Isolated from Rice Rhizosphere Soils from Southern India
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Insoluble Phosphate Solubilization by Bacterial Strains Isolated from Rice Rhizosphere Soils from Southern India

机译：从印度南部水稻根际土壤中分离的细菌菌株增溶不溶性磷酸盐

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Phosphates solubilizing bacterial strains were isolated from various rice- rhizosphere soils of southern peninsular region of India. Thirty efficient PSB isolates were selected from 226 colonies based on their ability to form clear zone on Pikovskaya?s agar medium. Phosphorus release and phosphatase activity were quantified using ELISA on 3rd, 5th and 7th day of incubation to determine the efficiency of the strains. The isolated phosphate solubilizing bacterial strains released high amount of phosphorus from tricalcium phosphate and ranged from 22.4 to 825.8 μg P mL-1 and the amount of phosphatase secreted into the medium ranged from 11.6 to 64 U. From the results we conclude that the efficiency of the strain to solubilize phosphate or release phosphorus depends on the specificity of the enzyme phosphatase than the amount of phosphatase released into the medium. The efficient strains isolated from rice rhizosphere soils were identified as Enterobacter, Micrococuss , Pseudomonas , Bacillus , Klebsiella and Serretia . Among all the strains, A4 strain ( Enterobacter aerogenes ) released high amount of phosphorus. All the isolated PSB were efficient phosphate solubilizers and can be used as bioinoculants to increase the available phosphorus in the soil for rice plant growth.
机译：从印度南部半岛地区的各种水稻根际土壤中分离了可溶解细菌菌株的磷酸盐。根据其在Pikovskaya琼脂培养基上形成透明区的能力，从226个菌落中选择了30种有效PSB分离株。在培养的第3、5和7天，使用ELISA对磷的释放和磷酸酶活性进行定量，以确定菌株的效率。分离出的可溶解磷酸盐的细菌菌株从磷酸三钙中释放出大量的磷，范围为22.4至825.8μgP mL -1 ，分泌到培养基中的磷酸酶的量为11.6至64U。结果我们得出结论，菌株溶解磷或释放磷的效率取决于酶磷酸酶的特异性，而不是释放到培养基中的磷酸酶的量。从水稻根际土壤中分离到的高效菌株被鉴定为肠杆菌，小球菌，假单胞菌，芽孢杆菌，克雷伯菌和沙雷氏菌。在所有菌株中，A4菌株（产气肠杆菌）释放出大量的磷。所有分离出的PSB都是有效的磷酸盐增溶剂，可以用作生物增菌剂，以增加土壤中水稻植物的有效磷。

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