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首页> 外文期刊>International Journal of Pharmaceutical and Biological Research >Development of Bioassay for a therapeutic Protein obtained from Recombinant Escherichia Coli
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Development of Bioassay for a therapeutic Protein obtained from Recombinant Escherichia Coli

机译：从重组大肠杆菌获得的治疗性蛋白质生物测定方法的开发

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The genetically matured streptokinase has been improved thrombolytic activity. Streptokinase was identified by SDS page and the Endotoxin level was found to be less than the level as mentioned in British Pharmacopeia. The percentage purity was found to be 99.1%. Recombinant Streptokinase gives more result which may be clinically symptomatic Purification and quantification protein was done by using HPLC and Western blot Activity is verified by Chromogenic end point assay. Advance technique like kinetic assay (LAL's method) had been used for determination of exact level of Endotoxin. Sterility test had been done before formulation by varying using Membrane method
机译：遗传成熟的链激酶已提高了溶栓活性。通过SDS页鉴定出链激酶，发现内毒素水平低于英国药​​典中所述的水平。发现纯度百分比为99.1％。重组链激酶可产生更多结果，可能是临床症状。使用HPLC进行纯化和定量蛋白，并通过生色终点检测法验证了Western blot活性。动力学分析（LAL法）等先进技术已用于确定内毒素的确切水平。使用膜法改变配方前已进行了无菌测试

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