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Atrazine Biodegradation in a Cisne Soil Exposed to a Major Spill

机译:泄漏严重的锡尼土壤中阿特拉津的生物降解

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Conventional soil tests, culture-based microbial methods, and the novel method of 15N-DNA stable isotope probing (SIP) were employed to illustrate atrazine biodegradation as related to the physiochemical properties of an atrazine-exposed Cisne soil. The soil exhibited enhanced atrazine degradation and apparently accumulated cyanuric acid. The soil showed elevated ambient concentrations of NO3-; however NO3- did not suppress atrazine degradation. Atrazine natural attenuation was limited by incomplete distribution through the unsaturated soil matrix. Approximately four moles of inorganic N derived from atrazine were detected for each mole of atrazine carbon mineralized, indicating that at least 80% of the atrazine N was released (less than 20% assimilated). 15N-DNA- SIP experiments were conducted using15N (ring)- and 15N-ethylamino-atrazine. The results of these experiments failed to establish a causal relationship between in-situ atrazine-degradation and enrichment of DNA associated with soil microorganisms. These results are likely due to isotopic dilution, either as a result of insufficient 15N assimilation or competition by other N sources. Further experiments using 13C-ethyl/isoproylamino-atrazine may yet establish the identities of organisms responsible for enhanced natural attenuation exhibited in the Cisne soil.
机译:传统的土壤测试,基于培养物的微生物方法以及15N-DNA稳定同位素探测(SIP)的新方法被用来说明r去津的生物降解与暴露于at去津的Cisne土壤的理化特性有关。土壤表现出增强的阿特拉津降解能力,并明显积累了氰尿酸。土壤显示出较高的NO3-浓度;但是,NO3-不能抑制阿特拉津的降解。阿特拉津的自然衰减受到不饱和土壤基质中不完全分布的限制。对于每摩尔矿化的at去津碳,检测到约四摩尔源自from去津的无机氮,表明至少释放了80%的at去津N(少于20%被同化)。使用15N(环)-和15N-乙基氨基-r去津进行15N-DNA- SIP实验。这些实验的结果未能建立原位阿特拉津降解与土壤微生物相关DNA富集之间的因果关系。这些结果可能是由于同位素稀释造成的,这可能是由于15N吸收不足或其他N源竞争造成的。使用13C-乙基/异丙基氨基-r去津的进一步实验可能仍可确定导致Cisne土壤中自然衰减增强的生物的身份。

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