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首页> 外文期刊>International Journal of TROPICAL DISEASE & Health >Comparative Study between Molecular Diagnosis and Different Diagnostic Methods of Schistosma haematobium in Gezira State Sudan
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Comparative Study between Molecular Diagnosis and Different Diagnostic Methods of Schistosma haematobium in Gezira State Sudan

机译：苏丹吉济拉州血吸虫血吸虫病的分子诊断和不同诊断方法的比较研究

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Over 650 million people globally are at risk of infection with Schistosomiasis, with more than 200 million people infected, and higher disease rates occur in children. Objectives: The aim of this study was to compare the Molecular diagnostic method with different diagnostic methods (urine filtration, haematuria ultrasound). Methods: Eighty three students of Quran School for boys in Radwan village, Gezira state were recruited. The mean age was 12.45±3.2 with a range between 6-20 years. Urine samples were collected. Different diagnostic methods were used. DNA was extracted from the eggs. Polymerase Chain Reaction was done for all samples. Results: Fifty-four percent of the cases were positive with urine filtration method, of whom microhaematuria was seen in 28%, while 61.4% were positive by ultrasound. Using polymerase chain reaction 73.3% of the samples were positive for Schistosoma haematobium . The comparison between the different diagnostic methods and gold standard urine filtration techniques showed that haematuria had a sensitivity of 42.2% and specificity of 89.4% with a significant association, (P0.05) which had a sensitivity of 71% and specificity of 50%, while PCR showed a sensitivity of 100% and specificity of 60.5%, showing a significant association between PCR and filtration techniques. Conclusion: The study concluded that PCR was 100% sensitivite, while microhaematuria method was highly specific 89%. Ultrasound had alower specificity as compared with other diagnostic tests. It is recommended that different diagnostic techniques should be applied according to the situation of the disease.
机译：全球有6.5亿人处于感染血吸虫病的危险中，超过2亿人受到感染，儿童的发病率更高。目的：本研究的目的是将分子诊断方法与不同的诊断方法（尿液过滤，血尿超声）进行比较。方法：招募了位于吉济拉州拉德万村的古兰经男孩学校的八十三名学生。平均年龄为12.45±3.2，范围为6-20岁。收集尿液样本。使用了不同的诊断方法。从卵中提取DNA。对所有样品进行聚合酶链反应。结果：54％的病例通过尿液过滤法阳性，其中微血尿的占28％，而超声检查的占61.4％。使用聚合酶链反应，血吸虫血吸虫阳性率为73.3％。不同诊断方法和金标准尿液过滤技术的比较显示，血尿的敏感性为42.2％，特异性为89.4％，具有显着相关性（P0.05），血尿的敏感性为71％，特异性为50％， PCR显示灵敏度为100％，特异性为60.5％，显示PCR与过滤技术之间存在显着关联。结论：该研究得出结论，PCR为100％敏化剂，而微血尿法的特异性为89％。与其他诊断测试相比，超声的特异性较低。建议根据疾病情况采用不同的诊断技术。

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