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首页> 外文期刊>International Journal of Pharmacy and Pharmaceutical Sciences >IN VITRO EVALUATION OF ANTIFUNGAL POTENTIAL AND ELECTRON MICROSCOPIC STUDIES OF BACILLUS AMYLOLIQUEFACIENS AGAINST ASPERGILLUS SPECIES
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IN VITRO EVALUATION OF ANTIFUNGAL POTENTIAL AND ELECTRON MICROSCOPIC STUDIES OF BACILLUS AMYLOLIQUEFACIENS AGAINST ASPERGILLUS SPECIES

机译：芽孢杆菌芽孢杆菌的抗菌潜力及电子显微镜研究。

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Objective: Bacteria are able to synthesize a wide range of metabolites with fungicidal properties. The present study focused on the in vitro evaluation of antifungal potential of amyloliquefaciens DSM-1067 against Aspergillus spp . and its electron microscopic studies. Methods: An invitro evaluation of antifungal activity of bacterial secreted and cellular proteins was determined by microbroth dilution, disc diffusion and spore germination inhibition assays (SGIA). The cytotoxicity of these bacterial proteins was determined by hemolytic assay, and the effect of Bacillus amyloliquefaciens DSM 1067 lysate proteins on Aspergillus fumigatus was visualized by scanning electron microscopy (SEM). Results: Bacillus amyloliquefaciens DSM-1067 lysates showed the highest activity by inhibiting the growth of A. fumigatus , A. flavus completely at a concentration of 31.25 μg/ml. In vitro toxicity experiments resulted that the lysate of B. amyloliquefaciens DSM-1067was non-toxic against human erythrocytes even at high concentrations. These findings thus emphasize its usefulness in the development of new antifungal therapies. SEM analysis demonstrated the in vitro inhibition of A. fumigatus growth by B. amyloliquefaciens DSM-1067cytosolic proteins leading to wrinkled hyphae, irregular branching patterns. It also showed disruption of conidiophores development. These cytological effects of B. amyloliquefaciens DSM-1067on the hyphal growth of A. fumigatus can explain its potent anti- Aspergillus activity. Conclusion: The present investigation revealed that B. amyloliquefaciens DSM-1067 lysate protein can act as a potential candidate for exploration in the development of effective and non-toxic treatments against Aspergillus induced diseases. Its effect on the development of conidiophores and hyphal growth are studied in the present study. Keywords: Anti- Aspergillus, Bacillus amyloliquefaciens, Toxicity, Aspergillus species, Scanning electron microscopy.
机译：目的：细菌能够合成具有杀真菌特性的多种代谢产物。本研究的重点是体外评价戊糖乳杆菌DSM-1067对曲霉的抑菌作用。及其电子显微镜研究。方法：通过微泡稀释，椎间盘扩散和孢子萌发抑制试验（SGIA），体外评估细菌分泌的和细胞蛋白的抗真菌活性。通过溶血测定法测定这些细菌蛋白的细胞毒性，并且通过扫描电子显微镜（SEM）观察到解淀粉芽孢杆菌DSM 1067裂解物蛋白对烟曲霉的影响。结果：解淀粉芽孢杆菌DSM-1067裂解物以31.25μg/ ml的浓度完全抑制烟曲霉，黄曲霉的生长而表现出最高的活性。体外毒性实验结果表明，解淀粉芽孢杆菌DSM-1067的裂解物即使在高浓度下也对人的红细胞无毒。因此，这些发现强调了其在开发新的抗真菌疗法中的有用性。 SEM分析表明，解淀粉芽孢杆菌DSM-1067胞浆蛋白在体外抑制了烟曲霉的生长，导致皱纹的菌丝，不规则的分支模式。它还显示了分生孢子发育的破坏。解淀粉芽孢杆菌DSM-1067对烟曲霉菌丝生长的这些细胞学作用可以解释其有效的抗曲霉活性。结论：本研究表明，解淀粉芽孢杆菌DSM-1067裂解物蛋白可作为开发潜在有效候选物的有效候选物，以抗曲霉菌引起的疾病。在本研究中研究了其对分生孢子的发展和菌丝生长的影响。关键词：抗曲霉，解淀粉芽孢杆菌，毒性，曲霉菌种，扫描电镜。

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