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首页> 外文期刊>International Journal of Pharmacy and Pharmaceutical Sciences >SCREENING OF ENDOPHYTIC BACTERIA PRODUCING ANTIFUNGAL ISOLATED FROM INDONESIA MEDICINAL PLANT, JAVA GINSENG (TALINUM TRIANGULARE) (JACQ.) WILLD
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SCREENING OF ENDOPHYTIC BACTERIA PRODUCING ANTIFUNGAL ISOLATED FROM INDONESIA MEDICINAL PLANT, JAVA GINSENG (TALINUM TRIANGULARE) (JACQ.) WILLD

机译：印度尼西亚人参（塔里木三角省）野生药用植物中分离出的产生内生细菌的内生细菌的筛选

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Objective: The objective of this research was to isolate and characterize endophytic bacteria from Talinum triangulare having antifungal activity. Methods: The endophytic bacteria were isolated from roots tissue of Talinum triangulare by surface sterilization method. The isolates were cultured in Trypticase Soybean agar and antagonist activities were evaluated by dual culture assay against Fusarium oxysporum, Trichoderma reesei and Candida albicans . For metabolite antifungal activities, bacterial isolates were grown for 4 d in TS broth at 35 °C under shaking condition. The antifungal activities of the supernatant extract were determined by using the disk agar diffusion. Polyketide synthase (PKS I) and NRPS genes fragments of all isolates were amplified. Results: The result reveals that 4 of 23 endophytic bacterial isolates demonstrated great antifungal potentiality against many tested fungi. Polyketide synthase (PKS I) and NRPS genes amplification were showed 10 and 4 of endophytic isolates detected harboring PKS type I and NRPS genes, respectively. In general, high frequencies of positive PCR amplification were obtained for PKS I (43.47%). Phylogenetic analyses based on the 16S rRNA gene sequence, morphological, physiological and biochemical showed that the isolates were identified as a member of genus Bacillus and Brevibacillus . Conclusion: These results indicated that the endophytic bacteria from java ginseng could be used as an alternative source of antifungal agents.
机译：目的：本研究的目的是分离和鉴定具有抗真菌活性的三角叶狼毒内生细菌。方法：采用表面灭菌法从三角叶楠的根组织中分离出内生细菌。分离株在胰蛋白酶大豆琼脂中培养，并通过双重培养法评估对尖孢镰刀菌，里氏木霉和白色念珠菌的拮抗活性。对于代谢物的抗真菌活性，将细菌分离株在35°C的振荡条件下于TS肉汤中生长4 d。通过使用圆盘琼脂扩散法测定上清液提取物的抗真菌活性。扩增了所有分离株的聚酮化合物合酶（PKS I）和NRPS基因片段。结果：结果表明，在23种内生细菌分离物中，有4种对许多测试真菌表现出极大的抗真菌潜力。聚酮化合物合酶（PKS I）和NRPS基因扩增显示，分别检测到10个和4个带有PKS I型和NRPS基因的内生细菌。通常，对于PKS I，获得了高频率的PCR阳性扩增结果（43.47％）。根据16S rRNA基因序列，形态，生理和生化指标的系统发育分析表明，分离株被鉴定为芽孢杆菌和短杆菌属的成员。结论：这些结果表明，人参爪哇的内生细菌可以用作抗真菌剂的替代来源。

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