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首页> 外文期刊>International Research Journal of Biological Sciences >Identification of Rice Kinesin 13A Interacting Proteins through Yeast Two-Hybrid Screening
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Identification of Rice Kinesin 13A Interacting Proteins through Yeast Two-Hybrid Screening

机译：通过酵母双杂交筛选鉴定水稻驱动蛋白13A相互作用蛋白

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By using full-length rice kinesin 13A protein (OsKIN13A) as a bait construct and rice cDNA library as a prey, we obtained several interacting proteins through yeast two-hybrid (Y2H) screening. These interacting proteins were selected by beta-galactose filter assay after growing on SD-TLH media containing 25 mM of 3-aminotriazole (3-AT). All of selected interacting proteins were confirmed by sequencing and annotated according to the rice genome database, and grouped into seven functional categories, i.e. cellular processes, transport and binding protein, metabolic activity, signal transduction, molecular chaperone and other category. This study suggests that OsKIN13A protein is associated with wide range of biological processes such as development, signaling and immunity.
机译：通过使用全长水稻驱动蛋白13A蛋白（OsKIN13A）作为诱饵构建体，并使用水稻cDNA库作为猎物，我们通过酵母双杂交（Y2H）筛选获得了几种相互作用的蛋白。在包含25 mM 3-氨基三唑（3-AT）的SD-TLH培养基上生长后，通过β-半乳糖过滤法选择这些相互作用的蛋白质。所有选择的相互作用蛋白均通过测序确认，并根据水稻基因组数据库进行注释，并分为七个功能类别，即细胞过程，转运和结合蛋白，代谢活性，信号转导，分子伴侣和其他类别。这项研究表明，OsKIN13A蛋白与广泛的生物学过程有关，例如发育，信号传导和免疫。

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