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Feather barbs as a good source of mtDNA for bird species identification in forensic wildlife investigations

机译：羽毛倒钩是用于法医野生动植物调查的鸟类物种鉴定的mtDNA的良好来源

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Background The ability to accurately identify bird species is crucial for wildlife law enforcement and bird-strike investigations. However, such identifications may be challenging when only partial or damaged feathers are available for analysis. Results By applying vigorous contamination controls and sensitive PCR amplification protocols, we found that it was feasible to obtain accurate mitochondrial (mt)DNA-based species identification with as few as two feather barbs. This minimally destructive DNA approach was successfully used and tested on a variety of bird species, including North American wild turkey (Meleagris gallopavo), Canada goose (Branta canadensis), blue heron (Ardea herodias) and pygmy owl (Glaucidium californicum). The mtDNA was successfully obtained from 'fresh' feathers, historic museum specimens and archaeological samples, demonstrating the sensitivity and versatility of this technique. Conclusions By applying appropriate contamination controls, sufficient quantities of mtDNA can be reliably recovered and analyzed from feather barbs. This previously overlooked substrate provides new opportunities for accurate DNA species identification when minimal feather samples are available for forensic analysis.
机译：背景技术准确识别鸟类的能力对于野生动物执法和鸟类打击调查至关重要。但是，当只有部分或损坏的羽毛可用于分析时，这种识别可能会很困难。结果通过应用严格的污染控制和灵敏的PCR扩增方案，我们发现用最少的两个羽毛倒钩获得基于线粒体（mt）DNA的准确物种识别是可行的。这种破坏性最小的DNA方法已成功用于多种鸟类，包括北美野生火鸡（Meleagris gallopavo），加拿大鹅（Branta canadensis），蓝鹭（Ardea herodias）和侏儒猫头鹰（Glaucidium californicum）。 mtDNA是从“新鲜”的羽毛，历史悠久的博物馆标本和考古样本中成功获得的，证明了该技术的敏感性和多功能性。结论通过采取适当的污染控制措施，可以可靠地从羽毛倒钩中回收和分析足够数量的mtDNA。当最少的羽毛样品可用于法医分析时，这种先前被忽视的底物为准确鉴定DNA种类提供了新的机会。

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