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首页> 外文期刊>Investigative Genetics >Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations
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Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations

机译：评估丁香假单胞菌病原菌胁迫对微生物法医调查中多位点可变数目串联重复分析和多位点序列分型的有效性的影响

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Background Crops in the USA are vulnerable to natural and criminal threats because of their widespread cultivation and lack of surveillance, and because of implementation of growing practices such as monoculture. To prepare for investigation and attribution of such events, forensic assays, including determination of molecular profiles, are being adapted for use with plant pathogens. The use of multi-locus variable number tandem repeat (VNTR) analysis (MLVA) and multi-locus sequence typing (MLST) in investigations involving plant pathogens may be problematic because the long lag periods between pathogen introduction and discovery of associated disease may provide enough time for evolution to occur in the regions of the genome employed in each assay. Thus, more information on the stability of the loci employed in these methods is needed. Results The MLVA fingerprints and MLST profiles were consistent throughout the experiment, indicating that, using a specific set of primers and conditions, MLVA and MLST typing systems reliably identify P.s. tomato DC3000. This information is essential to forensic investigators in interpreting comparisons between MLVA and MLST typing profiles observed in P.s. tomato isolates. Conclusions Our results indicate that MLVA and MLST typing systems, utilizing the specified primers and conditions, could be employed successfully in forensics investigations involving P.s. tomato. Similar experiments should be conducted in the field and with other high-consequence plant pathogens to ensure that the assays are reliable for pathogens infecting plants in their natural environment and for organisms that may display faster rates of mutation.
机译：背景技术由于在美国的农作物广泛耕种和缺乏监视，并且由于实施了诸如单作养殖等日益增长的实践，因此它们容易受到自然和刑事威胁。为了准备对此类事件进行调查和归因，法医化验（包括确定分子谱）正适合与植物病原体一起使用。在涉及植物病原体的调查中使用多位点可变数目串联重复序列（VNTR）分析（MLVA）和多位点序列分型（MLST）可能会出现问题，因为病原体引入与发现相关疾病之间的漫长时间间隔可能会提供足够的时间在每种测定中使用的基因组区域发生进化的时间。因此，需要更多有关这些方法中使用的基因座稳定性的信息。结果MLVA指纹图谱和MLST谱在整个实验过程中是一致的，这表明，使用一组特定的引物和条件，MLVA和MLST分型系统可以可靠地鉴定P.s。番茄DC3000。该信息对于法医调查人员解释在P.s中观察到的MLVA和MLST类型概况之间的比较至关重要。番茄分离株。结论我们的结果表明，利用指定的引物和条件的MLVA和MLST分型系统可成功用于涉及P.s的法医调查。番茄。应该在野外以及对其他高后果植物病原体进行类似的实验，以确保该测定法对于在自然环境中感染植物的病原体以及可能显示出更快突变速率的生物体是可靠的。

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