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首页> 外文期刊>Iranian journal of public health. >Detection of Extended Spectrum B-Lactamases in Urinary Isolates of Klebsiella pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage
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Detection of Extended Spectrum B-Lactamases in Urinary Isolates of Klebsiella pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage

机译：与Bla SHV ，Bla TEM 和Bla CTX-M 基因载体相关的肺炎克雷伯菌肺分离物中超广谱B-内酰胺酶的检测

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BackgroundResistance to contemporary broad-spectrum β-lactam antibiotics mediated by extended-spectrum β-lactamases (ESBLs) is increasing worldwide. Klebsiella pneumoniae, an important cause of nosocomial and community acquired urinary tract infections has rapidly become the most common ESBL producing organism. We examined ESBL production in urinary isolates of K. pneumoniae in relation to the presence of blaSHV, blaTEM and blaCTX-M genes.Methods:Antibiotic resistance of 51 clinical isolates of K. pneumoniae was determined to amoxicillin, amikacin, ceftazidime, cefotaxime, cefteriaxon, ceftizoxime, gentamicin, ciprofloxacin and nitrofurantoin by disc diffusion. Minimum inhibitory concentrations were also measured for ceftazidime, cefotaxime, cefteriaxon, ceftizoxime and ciprofloxacin. ESBL production was detected by the double disc synergy test and finally, presence of the blaSHV, blaTEM and blaCTX-M genes were shown using specific primers and PCR.Results:Disc diffusion results showed that 96.08 % of the isolates were resistant to amoxicillin followed by 78.43 % resistance to nitrofurantoin, 49.02 % to amikacin and ceftazidime, 41.17 % to ceftriaxone, 37.25% resistance to cefotaxime and ceftizoxime, and 29.42 % to gentamicin and ciprofloxacin. Both resistant and intermediately resistant organisms were resistant in MIC determinations. Twenty two isolates (43.14%) carried blaSHV, 18 (35.29%) had blaTEM and 16 (31.37%) harbored blaCTX-M genes. ESBL production was present in 14 isolates (27.45 %) of which, 3 did not harbor any of the 3 genes. Among the non-ESBL producers, 9 lacked all 3 genes and 2 carried them all.Conclusion:No relation was found between gene presence and ESBL expression.
机译：背景技术在世界范围内，对由广谱β-内酰胺酶（ESBLs）介导的当代广谱β-内酰胺抗生素的耐药性正在增加。肺炎克雷伯菌是医院和社区获得性泌尿道感染的重要原因，已迅速成为最常见的ESBL产生生物。我们检查了与blaSHV，blaTEM和blaCTX-M基因相关的肺炎克雷伯菌尿分离株中ESBL的产生。 ，头孢唑肟，庆大霉素，环丙沙星和呋喃妥因的扩散。还测定了头孢他啶，头孢噻肟，头孢特肟，头孢唑肟和环丙沙星的最小抑菌浓度。通过双盘协同试验检测ESBL的产生，最后，通过特异性引物和PCR显示blaSHV，blaTEM和blaCTX-M基因的存在。结果：圆盘扩散结果表明，分离株中96.08％对阿莫西林具有抗性，其次是对呋喃妥因的抗药性为78.43％，对丁胺卡那霉素和头孢他啶的抗药性为49.02％，对头孢曲松的抗药性为41.17％，对头孢噻肟和头孢唑肟的抗药性为37.25％，对庆大霉素和环丙沙星的抗药性为29.42％。抗性和中度抗性微生物在MIC测定中均具有抗性。带有blaSHV的22个分离株（43.14％），带有blaTEM的18个（35.29％）和带有blaCTX-M基因的16个（31.37％）。 ESBL的生产存在于14个分离株中（占27.45％），其中3个没有3个基因。在非ESBL生产者中，有9个缺乏全部3个基因，有2个携带全部。结论：基因存在与ESBL表达之间没有关系。

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