...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Iranian Journal of Immunology >Glycation of Human IgG Induces Structural Alterations Leading to Changes in its Interaction with Anti-IgG
【24h】

Glycation of Human IgG Induces Structural Alterations Leading to Changes in its Interaction with Anti-IgG

机译：人IgG的糖基化诱导结构改变，导致其与抗IgG相互作用的改变

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Glycation of proteins is a non-enzymatic spontaneous process that occurs in diabetes mellitus and aging, altering the structure and function of proteins. IgG undergoes glycation leading to changes in its reactivity to antigen and fixation of complement. ? Objective: This study aimed at revealing the effect of glycation on the interaction of IgG with anti-IgG using electroimmunoassay. ? Methods: Purified human IgG was glycated with different concentrations of glucose and different periods of treatment. Glycation was measured using thiobarbituric acid reaction. Glycated and non-glycated IgG were subjected to electroimmunoassay, and the height of the precipitated rings were measured and compared. ? Results: The results showed that IgG was glycated in vitro and the level of glycation was dependent on the glucose concentration and duration of treatment with glucose. The height of glycated IgG peaks formed in the electroimmunoassay was significantly lower than those of nonglycated IgG ( ? p 0.01). Conclusion: The results indicated that in vitro glycation of IgG leads to structural changes altering its mobility in the electroimmunoassay. Moreover, it suggests that this alteration may cause the weakness of its interaction with anti-IgG. This phenomenon may play a role in the susceptibility of diabetic patients to infection.
机译：背景：蛋白质的糖基化是非酶的自发过程，发生在糖尿病和衰老中，改变了蛋白质的结构和功能。 IgG进行糖基化作用，从而导致其对抗原的反应性变化和补体固定。 ？目的：本研究旨在通过电免疫分析揭示糖基化对IgG与抗IgG相互作用的影响。 ？方法：纯化的人IgG用不同浓度的葡萄糖和不同的治疗时间糖化。使用硫代巴比妥酸反应测量糖基化。对糖化和非糖化的IgG进行电免疫测定，并测量和比较沉淀环的高度。 ？结果：结果表明，IgG在体外糖基化，糖基化水平取决于葡萄糖浓度和葡萄糖治疗时间。电免疫测定中形成的糖化IgG峰的高度显着低于未糖化IgG的峰（Δp <0.01）。结论：结果表明，在免疫免疫测定中，IgG的体外糖基化会导致结构变化，从而改变其迁移率。此外，这表明这种改变可能导致其与抗IgG相互作用的弱点。这种现象可能在糖尿病患者对感染的易感性中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号