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首页> 外文期刊>Iranian Journal of Microbiology >A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens
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A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens

机译：常规技术的比较分析：逆转录酶聚合酶链反应（RT-PCR）和五种检测粪便标本中肠病毒的细胞系

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Background and objectives: Each year, Enteroviruses infect millions of people and cause different diseases. The agents are usually detected using cell culture. RD (Rhabdomyosarcoma) and L20B (L cells) are among the recommended cells by the World Health Organisation (WHO) for this purpose. Even though cell culture is the most common method used in diagnosing Enteroviruses in stool specimens, this particular method poses some problems, which include false positive or negative results, lack of a unique cell line for diagnosing all Enterovirus types in addition to being time consuming. For these reasons, an attempt was made to find better techniques of Enterovirus detection. RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) is a technique used in place of the cell culture method. In this study, the cell culture method was compared with RT-PCR for detection of Enteroviruses in stool specimens.Material and method: First, the chloroform treated stool samples were inoculated onto five cell lines, including RD, L20B, Hep-2 (Human Epidermoid carcinoma cell line), Vero (Verde Reno) and GMK (Green Monkey Kidney). The results were then compared with data from Enterovirus detection using the RT-PCR technique.Results and conclusion: The difference between RT-PCR and cell culture results was significant. Enteroviruses were detected in 24% of specimens using RT-PCR while cell lines could isolate Enteroviruses in just 14.4% of the samples.
机译：背景和目的：肠病毒每年感染数百万人，并引起不同的疾病。通常使用细胞培养物检测试剂。 RD（横纹肌肉瘤）和L20B（L细胞）是世界卫生组织（WHO）为此目的推荐的细胞。尽管细胞培养是粪便标本中诊断肠病毒的最常用方法，但这种特殊方法仍存在一些问题，包括假阳性或阴性结果，除了耗时之外，还缺乏用于诊断所有肠病毒类型的独特细胞系。由于这些原因，试图寻找更好的肠道病毒检测技术。 RT-PCR（逆转录酶聚合酶链反应）是一种代替细胞培养方法的技术。本研究将细胞培养方法与RT-PCR进行粪便标本中肠病毒的检测方法进行了比较。材料与方法：首先，将氯仿处理的粪便样品接种到RD，L20B，Hep-2（人类）等5种细胞系中表皮样癌细胞系），Vero（Verde Reno）和GMK（Green Monkey Kidney）。然后将结果与使用RT-PCR技术检测肠病毒的数据进行比较。结果与结论：RT-PCR与细胞培养结果之间存在显着差异。使用RT-PCR在24％的标本中检测到肠病毒，而细胞系仅在14.4％的样品中可以分离出肠病毒。

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