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首页> 外文期刊>Iranian Journal of Microbiology >Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins.
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Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins.

机译：分离自不同感染源的铜绿假单胞菌中毒力基因的分子鉴定和检测。

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Background and Objectives: Pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity. The aim of this study was to evaluate oprI, oprL and toxA genes for PCR identification of clinical P. aeruginosa. In order to find out any relation between special virulence factors and special manifestation of P. aeruginosa infections, we detected virulence factors among these isolates by PCR. Ribotyping was used to evaluate the clonal relationship between strains with the same genetic patterns of the genes studied.Materials and Methods: In this study, 268 isolates of P. aeruginosa were recovered from burn, wound and pulmonary tract infections. The prevalence of oprI, oprL, toxA, lasB, exoS and nan1 genes was determined by PCR. One hundred and four isolates were selected randomly to investigate clonal diversity of the isolates with ribotyping using SmaI.Results and Conclusions: All P. aeruginosa isolates in this study carried oprI, oprL and lasB genes. Difference between exoS prevalence in isolates from pulmonary tract and burn isolates was statistically significant. Prevalence of nan1 and toxA gene was significantly higher in pulmonary tract and burn isolates, respectively. Ribotyping showed that most of the isolates (87%) belonged to clone A and B.Detection of oprI, oprL and toxA genes by PCR is recommended for molecular identification of P. aeruginosa. Determination of different virulence genes of P. aeruginosa isolates suggests that they are associated with different levels of intrinsic virulence and pathogenicity. Ribotyping showed that strains with the same genetic patterns of the genes do not necessarily have similar ribotype patterns.
机译：背景与目的：铜绿假单胞菌具有多种可能导致其致病性的毒力因子。这项研究的目的是评估oprI，oprL和toxA基因用于临床铜绿假单胞菌的PCR鉴定。为了找出铜绿假单胞菌感染的特殊毒力因子与特殊表现之间的关系，我们通过PCR检测了这些分离株中的毒力因子。核糖体分型用于评估具有相同基因遗传模式的菌株之间的克隆关系。材料与方法：在这项研究中，从烧伤，伤口和肺部感染中回收了268株铜绿假单胞菌。通过PCR确定oprI，oprL，toxA，lasB，exoS和nan1基因的患病率。结果：结论：本研究中所有铜绿假单胞菌菌株均带有oprI，oprL和lasB基因，随机选择了104个菌株进行核糖分型的克隆多样性研究。肺部分离株和烧伤分离株的exoS患病率之间的差异具有统计学意义。 nan1和toxA基因的患病率分别在肺部和烧伤隔离株中显着升高。核型分析表明，大多数分离株（87％）属于克隆A和B。建议通过PCR检测oprI，oprL和toxA基因来鉴定铜绿假单胞菌。铜绿假单胞菌分离物不同毒力基因的确定表明它们与不同水平的内在毒力和致病性有关。核型分析表明，具有相同基因遗传模式的菌株不一定具有相似的核型。

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