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首页> 外文期刊>Iranian Journal of Microbiology >Genetic evaluation of Locus of enterocyte effacement pathogenicity island (LEE) in Enteropathogenic Escherichia coli isolates (EPEC).
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Genetic evaluation of Locus of enterocyte effacement pathogenicity island (LEE) in Enteropathogenic Escherichia coli isolates (EPEC).

机译：肠致病性大肠杆菌分离株（EPEC）中肠上皮细胞致病性岛（LEE）基因座的遗传评估。

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Background and Objectives: Enteropathogenic Escherichia coli (EPEC) divided into two groups typical and atypical (aspect). The main virulence genes are located in a pathogenicity island called LEE (Locus of Enterocyte Effacement). LEE frequently inserted in tRNA genes of selC, pheU and pheV in the bacterial chromosome. tEPEC and aEPEC strains have some differences in their pathogenicity. The purpose of this was to investigate the possible differences between tEPEC and aEPEC strains according to the virulence genes encoding by LEE and their relation to insertion sites.Materials and Methods: In this study 130 E. coli isolates confirmed by biochemical analysis from diarrheal patients, were evaluated for EPEC pathotype by PCR. All EPEC strains tested for presence of some LEE encoded virulence genes and sites of LEE insertion by PCR method.Results: Among 50 strains of EPEC 28 (56%) and 22 (44%) were typical and atypical strains respectively. 19 strains (30%) showed insertion in selC, 7 (14%) in pheU, 4 (8%) in pheV, 8 (16%) in pheU and pheV, 1 (2%) in selC and pheU, 6 (12%) in pheV, pheU and selC and 5 (10%) had no insertion in these sites. Moreover, spa (n = 8, 16%), espB (n = 16, 32%), espD (n =18, 36%), espF (n = 8, 16%), espG (n = 13, 26%), espH (n = 12, 24%), map (n = 11, 32%) and tir (n = 4, 8%) were present among the strains.Conclusion: Results showed that most of the virulence genes are present in tEPEC isolates. However, aEPEC isolates may acquire other virulence factors. The majority of tEPEC strains showed insertion at selC and aEPEC strains in pheV and pheU.
机译：背景与目的：肠致病性大肠杆菌（EPEC）分为典型和非典型（方面）两类。主要毒力基因位于称为LEE（肠上皮细胞形成部位）的致病岛上。 LEE经常插入细菌染色体中的selC，pheU和pheV的tRNA基因中。 tEPEC和aEPEC菌株的致病性有所不同。目的是根据LEE编码的毒力基因及其与插入位点的关系，研究tEPEC和aEPEC菌株之间的可能差异。材料与方法：本研究通过腹水患者的生化分析确定了130株大肠杆菌，通过PCR评估EPEC型。所有EPEC菌株通过PCR方法检测了一些LEE编码的毒力基因的存在和LEE插入位点。结果：在50株EPEC菌株中，典型菌株为28（56％），非典型菌株为22（44％）。 19株（30％）显示插入selC中，pheU中插入7（14％），pheV中插入4（8％），pheU和pheV中插入8（16％），selC和pheU中插入1（2％），6（12 ％）在pheV，pheU和selC中，其中5个（10％）没有插入这些位点。此外，spa（n = 8、16％），espB（n = 16、32％），espD（n = 18、36％），espF（n = 8、16％），espG（n = 13、26％） ），espH（n = 12，24％），map（n = 11，32％）和tir（n = 4，8％）存在于菌株中。结论：结果表明，大多数毒力基因都存在于该菌株中。 tEPEC分离株。但是，aEPEC分离株可能会获得其他毒力因子。大多数tEPEC菌株在pheV和pheU中插入selC，而aEPEC菌株插入。

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