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首页> 外文期刊>Eastern Mediterranean Health Journal: Al-Magallat al-Sihhiyyat li-Sarq al-Mutawassit >Caractérisation génétique du sous-groupe Maculipennis (Diptera : Culicidae) au Maroc : un outil fondamental pour lutter contre le paludisme
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Caractérisation génétique du sous-groupe Maculipennis (Diptera : Culicidae) au Maroc : un outil fondamental pour lutter contre le paludisme

机译:摩洛哥Maculipennis子群(双翅目:Culicidae)的遗传特征:抗击疟疾的基本工具

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The Anopheles maculipennis complex has been implicated in the transmission of malaria, whichwas endemic in Morocco until 2010. Climate change, intensification of international travel and the permanentpresence of the vector increase the risk of malaria re-emergence. Using polymerase chain reaction assay (PCR),this study attempts to identify the possible presence of the Maculipennis complex in several areas of Morocco,based on the concept of risk. Eighty-six mosquito specimens were analyzed using PCR amplification of theITS2 (Internal Transcribed Spacer 2) sequence of ribosomal DNA. The ITS2 sequences of the mosquitoes werecompared to those of the 7 species of the Maculipennis complex available in GenBank. The results obtainedconfirm the presence of Anopheles labranchiae. In order to enable technology transfer, DNA extraction andamplification steps are presented for the first time in detail, given the fact that the technique is still unknown toseveral Moroccan peripheral laboratories.
机译:疟疾按蚊综合体一直与疟疾的传播有关,该疾病在摩洛哥一直流行到2010年。气候变化,国际旅行的加剧和媒介的永久存在增加了疟疾再次出现的风险。本研究使用聚合酶链反应分析(PCR),尝试根据风险概念来确定Maculipennis复合物在摩洛哥几个地区的可能存在。使用PCR扩增核糖体DNA的ITS2(内部转录间隔区2)序列分析了86个蚊子标本。蚊子的ITS2序列与GenBank中Maculipennis复合体的7种物种的ITS2序列相比。获得的结果证实了拉氏按蚊的存在。为了使技术转让,鉴于该技术对几个摩洛哥外围实验室仍是未知的事实,首次详细介绍了DNA提取和扩增步骤。

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