...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Iranian Journal of Neurology >The effects of trypsin on rat brain astrocyte activation
【24h】

The effects of trypsin on rat brain astrocyte activation

机译：胰蛋白酶对大鼠脑星形胶质细胞活化的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundAstrocytes are cells within the central nervous system which are activated in a wide spectrum of infections, and autoimmune and neurodegenerative diseases. In pathologic states, they produce inflammatory cytokines, chemokines, and nitric oxide (NO), and sometimes they induce apoptosis. Their protease-activated receptors (PARs) can be activated by proteases, e.g. thrombin and trypsin, which are important in brain inflammation. The current study aimed to investigate the effects of different concentrations of trypsin (1 to 100U/ml) on cultured astrocytes.MethodsIn the present study, two-day rat infants’ brains were isolated and homogenized after meninges removal, then cultivated in DMEM + 10% FBS medium. 10 days later, astrocytes were harvested and recultivated for more purification (up to 95%), using Immunocytochemistry method, in order to be employed for tests. They were affected by different concentrations of trypsin (1, 5, 10, 15, 20, 40, 60, 80, and 100 U/ml). To reveal the inflammation progress, NO concentrations (the Griess test) were assessed after 24 and 48 hours.ResultsThe results showed that trypsin concentration up to 20 U/ml caused a significant increase in NO, in a dose-dependent manner, on cultured astrocytes (P < 0.001). Trypsin 20 U/ml increased NO production fivefold the control group (P < 0.001). At higher concentrations than 20 U/ml, NO production diminished (P < 0.001). At 100 U/ml, NO production was less than the control group (P < 0.001).ConclusionInflammatory effects of trypsin 5-20 U/ml are probably due to the stimulation of astrocytes’ PAR-2 receptors and the increasing of the activation of NF-κB, PKC, MAPKs. Stimulation of astrocytes’ PAR-2 receptors causes an increase in iNOS activation which in turn leads to NO production. However, higher trypsin concentration possibly made astrocyte apoptosis; therefore, NO production diminished. These assumptions need to be further investigated.
机译：背景星形胶质细胞是中枢神经系统中的细胞，在多种感染，自身免疫和神经退行性疾病中被激活。在病理状态下，它们会产生炎性细胞因子，趋化因子和一氧化氮（NO），有时还会诱导细胞凋亡。它们的蛋白酶激活的受体（PAR）可以被蛋白酶激活。凝血酶和胰蛋白酶，在脑部炎症中很重要。目前的研究旨在研究不同浓度的胰蛋白酶（1至100U / ml）对培养的星形胶质细胞的影响。方法在本研究中，将去除脑膜的两日龄大鼠婴儿的大脑分离并匀浆，然后在DMEM + 10中培养％FBS培养基。 10天后，使用免疫细胞化学方法收集星形胶质细胞并再培养以进行更多纯化（高达95％），以便用于测试。他们受到不同浓度的胰蛋白酶（1、5、10、15、20、40、60、80和100 U / ml）的影响。为了揭示炎症进展，在24和48小时后评估了NO浓度（Griess试验）。结果结果表明，高达20 U / ml的胰蛋白酶浓度以剂量依赖的方式引起培养的星形胶质细胞NO的显着增加。 （P <0.001）。胰蛋白酶20 U / ml将NO生成量增加到对照组的五倍（P <0.001）。在高于20 U / ml的浓度下，NO生成减少（P <0.001）。在100 U / ml时，NO的产生少于对照组（P <0.001）。结论胰蛋白酶5-20 U / ml的炎症作用可能是由于星形胶质细胞的PAR-2受体的刺激和活化的增加。 NF-κB，PKC，MAPKs。刺激星形胶质细胞的PAR-2受体会导致iNOS激活的增加，进而导致NO的产生。但是，较高的胰蛋白酶浓度可能会使星形胶质细胞凋亡。因此，没有产量减少。这些假设需要进一步研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号