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首页> 外文期刊>EBioMedicine >A Rapid Zika Diagnostic Assay to Measure Neutralizing Antibodies in Patients
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A Rapid Zika Diagnostic Assay to Measure Neutralizing Antibodies in Patients

机译：一种快速的Zika诊断测定法，可测量患者的中和抗体

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The potential association of microcephaly and other congenital abnormalities with Zika virus (ZIKV) infection during pregnancy underlines the critical need for a rapid and accurate diagnosis. Due to the short duration of ZIKV viremia in infected patients, a serologic assay that detects antibody responses to viral infection plays an essential role in diagnosing patient specimens. The current serologic diagnosis of ZIKV infection relies heavily on the labor-intensive Plaque Reduction Neutralization Test (PRNT) that requires more than one-week turnaround time and represents a major bottleneck for patient diagnosis. To overcome this limitation, we have developed a high-throughput assay for ZIKV and dengue virus (DENV) diagnosis that can attain the ''gold standard'' of the current PRNT assay. The new assay is homogeneous and utilizes luciferase viruses to quantify the neutralizing antibody titers in a 96-well format. Using 91 human specimens, we showed that the reporter diagnostic assay has a higher dynamic range and maintains the relative specificity of the traditional PRNT assay. Besides the improvement of assay throughput, the reporter virus technology has also shortened the turnaround time to less than two days. Collectively, our results suggest that, along with the viral RT-PCR assay, the reporter virus-based serologic assay could be potentially used as the first-line test for clinical diagnosis of ZIKV infection as well as for vaccine clinical trials.
机译：妊娠期间小头畸形和其他先天性异常与寨卡病毒（ZIKV）感染的潜在关联强调了对快速，准确诊断的迫切需求。由于感染患者中ZIKV病毒血症的持续时间较短，因此检测抗体对病毒感染反应的血清学检测方法在诊断患者标本中起着至关重要的作用。当前ZIKV感染的血清学诊断在很大程度上依赖于劳动强度大的减少斑块中和试验（PRNT），该试验需要超过一周的周转时间，并且代表了患者诊断的主要瓶颈。为了克服这一限制，我们开发了一种用于ZIKV和登革热病毒（DENV）诊断的高通量检测方法，可以达到当前PRNT检测方法的“金标准”。新的测定是均质的，并利用萤光素酶病毒以96孔的形式定量中和抗体的效价。我们使用91个人类标本，表明报告者诊断测定具有更高的动态范围，并保持了传统PRNT测定的相对特异性。除了提高分析通量外，报告病毒技术还将周转时间缩短到不到两天。总的来说，我们的结果表明，连同病毒RT-PCR测定法一样，基于报告基因病毒的血清学测定法可潜在地用作ZIKV感染的临床诊断以及疫苗临床试验的一线检测。

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