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首页> 外文期刊>EBioMedicine >HnRNP A1 is Involved in Deep Vein Thrombosis Patients with Behcet's Disease
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HnRNP A1 is Involved in Deep Vein Thrombosis Patients with Behcet's Disease

机译：HnRNP A1参与白塞氏病的深静脉血栓形成患者

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Objective: The aim of this study was to verify the hypothesis originated from bioinformatics and literature reviews that hnNRP A1 may be a new immune target of Behcet's disease (BD). Methods: First, bioinformatics was used to show the correlation between hnRNP A1 and A2/B1 in amino acid sequences and three dimensional structures. Second, hnRNP A1 was expressed, purified, and immunologically confirmed by systematic immunology methods including: Western blotting, immunoprecipitation and Dot-ELISA. Then, ELISA was used to screen the anti-hnRNP A1 autoantibodies in newly confirmed clinical samples and the clinical significance was compared between anti-hnRNP A1 antibody positive and negative groups. Finally, the endothelial cells antigen profile of one anti-hnRNP A1 antibody positive BD patient was detected using immunoprecipitation with liquid chromatography tandem mass spectrometry (LC-TMS). Results: In total 720 subjects enrolled and tested in this study. Our results demonstrated hnRNP A1 as a new immune target of BD. The reactivity of BD serum IgG antibodies against hnRNP A1 was significantly higher than healthy controls (P<0.0001), and deep vein thrombosis (DVT) showed a significant higher in the anti-hnRNP A1 antibodies positive group (P<0.05).
机译：目的：本研究的目的是验证源自生物信息学的假说和文献综述，即hnNRP A1可能是贝塞特病（BD）的新免疫靶标。方法：首先，利用生物信息学方法来显示hnRNP A1和A2 / B1在氨基酸序列和三维结构中的相关性。其次，通过系统免疫学方法表达，纯化并通过免疫学方法确认hnRNP A1，包括：Western印迹，免疫沉淀和Dot-ELISA。然后，使用ELISA法在新确诊的临床样品中筛选抗hnRNP A1自身抗体，并比较抗hnRNP A1抗体阳性和阴性组的临床意义。最后，使用液相色谱串联质谱（LC-TMS）进行免疫沉淀，检测了一名抗hnRNP A1抗体阳性BD患者的内皮细胞抗原谱。结果：总共720名受试者参加了这项研究并进行了测试。我们的结果证明hnRNP A1是BD的新免疫靶标。 BD血清IgG抗体对hnRNP A1的反应性显着高于健康对照组（P <0.0001），而深静脉血栓形成（DVT）在抗hnRNP A1抗体阳性组中显着更高（P <0.05）。

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