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首页> 外文期刊>Iranian Journal of Microbiology >Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates
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Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates

机译：香芹酚对耐氟康唑的白色念珠菌分离株分泌的天冬氨酰蛋白酶1-3的表达的抑制作用

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Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug.Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment.Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates.Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound.Keywords: Carvacrol, SAP1-3, Candida albicans, Fluconazole-resistant, Echinophora platyloba
机译：背景与目的：分泌天冬氨酰蛋白酶（SAP）是念珠菌发病的主要毒力因子之一。该酶由至少十个基因的家族编码。在这些基因中，SAP1-3在粘膜感染中的作用是显而易见的。本研究旨在探讨白念珠菌分离株SAP1-3基因在Echinophora platyloba提取物，香芹酚和卡泊芬净药物治疗后的表达情况。材料与方法：获得并培养68例疑似阴道炎妇女的阴道样本。利用表型和基因分型方法鉴定了白色念珠菌。用分光光度法研究阴道样品中SAP蛋白的存在，并用SDS-PAGE确定其蛋白组成。进行实时荧光定量PCR，以检测白chin棘皮提取物，香芹酚和卡泊芬净的亚抑制浓度对处理前后SAP1-3基因表达的影响。结果：白色念珠菌为丰富物种，占59.6％，且差异很大。所有阴道样品中都存在SAP的量，这高于克鲁斯假丝酵母菌株。估计白色念珠菌样品中SAP的蛋白质组成为大约45 kDa的分子量。抗FLU分离株中总SAP的mRNA水平（P = 0.01）高于FLU易感分离株（P = 0.07）。研究结果表明，香芹酚可有效降低SAP1-3的表达，对抗FLU的分离株具有特别的作用。结论：香芹酚含有香精油（香芹酚）;因此，它可以被认为是另一种有效的抗真菌化合物。关键字：香芹酚，SAP1-3，白色念珠菌，耐氟康唑，鸭嘴兽

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