...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>EBioMedicine >Bacterial viability in faecal transplants: Which bacteria survive?
【24h】

Bacterial viability in faecal transplants: Which bacteria survive?

机译：粪便移植中的细菌生存力：哪些细菌可以存活？

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The therapeutic potential of faecal microbiota transplantation (FMT) is under investigation for a range of inflammatory conditions. While mechanisms of benefit are poorly understood, most models rely on the viability of transplanted microbes. We hypothesised that protocols commonly used in the preparation of faecal transplants will substantially reduce the number, diversity and functional potential of viable microbes. Methods Stools from eight screened donors were processed under strict anaerobic conditions, in ambient air, and freeze-thawed. Propidium monoazide (PMA) sample treatment was combined with quantitative PCR, 16S rRNA gene amplicon sequencing and short-chain fatty acid (SCFA) analysis to define the viable microbiota composition and functional potential. Findings Approximately 50% of bacterial content of stool processed immediately under strict anaerobic conditions was non-viable. Homogenisation in ambient air or freeze-thaw reduced viability to 19% and 23% respectively. Processing of samples in ambient air resulted in up to 12-fold reductions in the abundance of important commensal taxa, including the highly butyrogenic species Faecalibacterium prausnitzii , Subdoligranulum variable , and Eubacterium hallii . The adverse impact of atmospheric oxygen exposure on the capacity of the transplanted microbiota to support SCFA biosynthesis was demonstrated by significantly reduced butyrate and acetate production by faecal slurries processed in ambient air. In contrast, while reducing overall levels of viable bacteria, freeze-thaw did not significantly alter viable microbiota composition. Interpretation The practice of preparing material for faecal transplantation in ambient air profoundly affects viable microbial content, disproportionately reducing the abundance of anaerobic commensals and the capacity for biosynthesis of important anti-inflammatory metabolites. Fund This work was supported by the South Australian Health and Medical Research Institute. LP is supported by a scholarship from the Flinders Foundation. GR is supported by a Matthew Flinders Research Fellowship.
机译：背景技术粪便微生物群移植（FMT）的治疗潜力正在研究各种炎症状况。尽管人们对收益的机制了解甚少，但大多数模型都依赖于移植微生物的生存力。我们假设，通常用于准备粪便移植的方案将大大减少活菌的数量，多样性和功能潜力。方法在严格的厌氧条件下，在环境空气中，对来自八名筛选供体的粪便进行处理，然后冻融。单叠氮化丙锭（PMA）样品处理与定量PCR，16S rRNA基因扩增子测序和短链脂肪酸（SCFA）分析相结合，以确定可行的微生物群组成和功能潜力。发现在严格的厌氧条件下立即处理的粪便中大约50％的细菌含量是不可行的。在环境空气中均质化或冻融将活力分别降低到19％和23％。在环境空气中处理样品会导致重要的共生类群的丰度降低多达12倍，其中包括高度产热菌种Faecalibacterium prausnitzii，Subdoligranulumum变种和Halleus alii。暴露在大气中的氧气对移植的微生物群支持SCFA生物合成的能力的不利影响通过在环境空气中处理的粪便显着降低了丁酸和乙酸盐的产生来证明。相反，在降低活菌的总体水平的同时，冻融并没有显着改变活菌的组成。解释准备在环境空气中进行粪便移植的材料的做法会深刻影响可行的微生物含量，不成比例地降低厌氧菌含量和重要的抗炎代谢产物的生物合成能力。基金这项工作得到了南澳大利亚卫生与医学研究所的支持。 LP由Flinders基金会提供的奖学金支持。 GR由Matthew Flinders研究奖学金支持。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号