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首页> 外文期刊>Ecology and Evolution >Seeing is believing? Comparing plant–herbivore networks constructed by field co‐occurrence and DNA barcoding methods for gaining insights into network structures
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Seeing is believing? Comparing plant–herbivore networks constructed by field co‐occurrence and DNA barcoding methods for gaining insights into network structures

机译：眼见为实？比较通过现场共现和DNA条形码方法构建的植物-草食动物网络，以深入了解网络结构

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摘要

Plant–herbivore interaction networks provide information about community organization. Two methods are currently used to document pairwise interactions among plants and insect herbivores. One is the traditional method that collects plant–herbivore interaction data by field observation of insect occurrence on host plants. The other is the increasing application of newly developed molecular techniques based on DNA barcodes to the analysis of gut contents. The second method is more appealing because it documents realized interactions. To construct complete interaction networks, each technique of network construction is urgent to be assessed. We addressed this question by comparing the effectiveness and reliability of the two methods in constructing plant–Lepidoptera larval network in a 50?ha subtropical forest in China. Our results showed that the accuracy of diet identification by observation method increased with the number of observed insect occurrences on food plants. In contrast, the molecular method using three plant DNA markers were able to identify food residues for 35.6% larvae and correctly resolved 77.3% plant (diet) species. Network analysis showed molecular networks had threefold more unique host plant species but fewer links than the traditional networks had. The molecular method detected plants that were not sampled by the traditional method, for example, bamboos, bryophytes and lianas in the diets of insect herbivores. The two networks also possessed significantly different structural properties. Our study indicates the traditional observation of co‐occurrence is inadequate, while molecular method can provide higher species resolution of ecological interactions.
机译：植物－草食动物互动网络提供有关社区组织的信息。当前使用两种方法来记录植物和昆虫食草动物之间的成对相互作用。一种方法是通过现场观察寄主植物上昆虫的发生来收集植物—草食动物相互作用数据的传统方法。另一个是基于DNA条码的新开发的分子技术在肠内容物分析中的应用不断增加。第二种方法更具吸引力，因为它记录了已实现的交互。为了构建完整的交互网络，迫切需要评估每种网络构建技术。通过比较两种方法在中国50公顷亚热带森林中建立植物鳞翅目幼虫网络的有效性和可靠性，我们解决了这个问题。我们的结果表明，通过观察方法识别饮食的准确性随着在食物植物上观察到的昆虫发生次数的增加而提高。相比之下，使用三种植物DNA标记物的分子方法能够鉴定35.6％的幼虫的食物残留，并正确解析了77.3％的植物（饮食）物种。网络分析表明，分子网络的独特寄主植物种类比传统网络多三倍，而链接却更少。分子方法检测到未用传统方法取样的植物，例如昆虫食草动物饮食中的竹子，苔藓植物和藤本植物。这两个网络还具有明显不同的结构特性。我们的研究表明，对共现的传统观察是不够的，而分子方法可以为生态相互作用提供更高的物种分辨率。

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