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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Detection of circulating tumor cells by nested RT-PCR targeting EGFR/CEA/CK20mRNAs in colorectal carcinoma patients
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Detection of circulating tumor cells by nested RT-PCR targeting EGFR/CEA/CK20mRNAs in colorectal carcinoma patients

机译：巢式RT-PCR靶向EGFR / CEA / CK20mRNAs检测大肠癌患者循环肿瘤细胞

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Background EGFR is involved in the epidermal growth factors pathway that regulates cellular processes and is associated with the development of many types of cancer including colorectal cancer. Molecular methods with high sensitivity such as nested polymerase chain reaction (PCR) based assays have been used to search for tumor cell specific markers. This study aimed to detect the circulating EGFRmRNA expressing tumor cells and its diagnostic value in colorectal cancer compared with that of known markers of circulating cancer cells CEA and CK20. Subjects and methods This study included 36 patients diagnosed as having colon cancer of different stages and 18 matched healthy controls. The staging was carried out according to the TNM classification. We used nested RT-PCR-based reverse transcription PCR assay for the detection of circulating cancer cells in the peripheral blood. Results The blood samples from the colon cancer patients showed detection of EGFR in 15/36 patients (41.7%); CEAmRNA in 22/36 patients (61.1%) and CK20mRNA in 24/36 patients (66.7%). No evidence of EGFR mRNA expression in any of the samples used as controls. 3/18 (16.7%) and 4/18 (22.2%) of healthy controls gave a positive result of CEA/CK20mRNAs. There was a statistically significant difference in the prevalence of EGFR/CEA and CK20mRNAs expression between the early disease group (stage I and II) and the advanced disease group (stage III and IV) ( P <0.01). Colon cancer patients with a high level of serum CEA exhibited detectable concentrations of EGFR and CEA and CK20mRNAs more often than those with a low serum CEA level, there is significant difference ( P <0.01). Conclusion EGFR assay might represent a suitable marker for detection of circulating tumor cells in colon cancer patients. CEA and CK20mRNAs are significantly more frequently detected in colon cancer patients than in healthy controls supports the hypothesis that they are promising complementary markers for CRC diagnosis. The assessment of multiple molecular tumor markers improved the sensitivity in detecting circulating tumor cells but due to limited specificity; identification and validation of genes and proteins implicated in metastatic processes need to be further investigated.
机译：背景技术EGFR参与调节细胞过程的表皮生长因子途径，并与包括结直肠癌在内的多种癌症的发展有关。具有高灵敏度的分子方法，例如基于巢式聚合酶链反应（PCR）的测定法，已被用于寻找肿瘤细胞特异性标志物。这项研究旨在检测循环中表达EGFRmRNA的肿瘤细胞及其与大肠癌细胞CEA和CK20的已知标记物相比的诊断价值。受试者和方法这项研究包括36位被诊断患有不同阶段结肠癌的患者和18位匹配的健康对照。分期是根据TNM分类进行的。我们使用基于嵌套式RT-PCR的逆转录PCR分析法检测外周血中循环的癌细胞。结果结肠癌患者的血液样本中有15/36例患者检测到EGFR（41.7％）。 CEAmRNA在22/36例患者中占61.1％，CK20mRNA在24/36例患者中占66.7％。在用作对照的任何样品中均未发现EGFR mRNA表达的证据。健康对照组的3/18（16.7％）和4/18（22.2％）给出了CEA / CK20mRNA的阳性结果。在早期疾病组（I和II期）和晚期疾病组（III和IV期）之间，EGFR / CEA和CK20mRNA的表达率存在统计学差异（P <0.01）。血清CEA水平高的结肠癌患者比血清CEA水平低的患者更经常检测到EGFR，CEA和CK20mRNA的浓度，差异有统计学意义（P <0.01）。结论EGFR检测可能是检测结肠癌患者循环肿瘤细胞的合适标志物。 CEA和CK20mRNA在结肠癌患者中的检出率显着高于健康对照组，这支持以下假设：它们是有希望用于CRC诊断的互补标记。对多种分子肿瘤标志物的评估提高了检测循环肿瘤细胞的敏感性，但由于特异性有限;涉及转移过程的基因和蛋白质的鉴定和验证需要进一步研究。

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