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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Correlation between the RhD genotyping and RhD serotyping in isoimmunized pregnancies
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Correlation between the RhD genotyping and RhD serotyping in isoimmunized pregnancies

机译：同种免疫的孕妇中RhD基因型与RhD血清型之间的相关性

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Alloimmunisation was one of the most important causes of perinatal mortality and morbidity by the middle of the last century. The objective of the present study was to investigate the presence of the RHD gene in fetal cells (amniocytes) obtained from amniotic fluid by genotyping to compare it with the RhD serotyping. Also to correlate the presence of RhD gene with the neonatal outcome. This work was carried out at Maternity hospital and Medical Genetics center, while PCR testing was done at the Medical Research center, Faculty of Medicine, Ain Shams University in the period from 2008 to 2010. The present study included recruiting of 20 RhD negative (sensitized to the RhD antigen) pregnant mothers. The entire study group was subjected to complete general, obstetric and a detailed obstetric ultrasonographic examination. Rh typing and indirect Coomb’s test were also done. Amniocentesis was performed with a 20-gauge needle under continuous ultrasound guidance. RhD serotyping of the fetuses showed that, 14 fetuses (70%) were positive and six fetuses (30%) were negative. While using RhD gentyping 13 cases (65%) were positive and seven cases (35%) were negative ( P value=0.002). Among fetuses positive for RhD genotyping six fetuses (46%) had received postnatal treatment, while among fetuses negative for RhD genotyping, neither of them had received postnatal treatment ( P value=0.032), which is statistically significant. From the present study we can conclude that, the identification of an antigen-negative fetus on the basis of the blood group genotype provides significant advantages in managing the pregnancy at risk for HDFN.
机译：到上个世纪中叶，同种免疫是围产期死亡率和发病率的最重要原因之一。本研究的目的是通过基因分型来研究从羊水获得的胎儿细胞（羊细胞）中RHD基因的存在，并将其与RhD血清分型进行比较。还可以将RhD基因的存在与新生儿预后相关联。这项工作是在2008年至2010年期间在孕妇医院和医学遗传学中心进行的，而PCR测试是在艾因谢姆斯大学医学院的医学研究中心进行的。本研究包括招募20例RhD阴性（致敏的）对RhD抗原）的孕妇。整个研究组均接受了全面的普通，产科和详细的产科超声检查。 Rh输入法和间接Coomb的测试也已完成。在连续超声引导下用20号针进行羊膜穿刺术。胎儿的RhD血清分型显示，有14胎（70％）为阳性，有6胎（30％）为阴性。在使用RhD基因分型时，有13例（65％）为阳性，有7例（35％）为阴性（P值= 0.002）。在RhD基因型阳性的胎儿中，有6名（46％）接受了产后治疗，而RhD基因型阴性的胎儿均没有接受过产后治疗（P值= 0.032），具有统计学意义。从本研究中我们可以得出结论，基于血型基因型鉴定抗原阴性胎儿在管理有HDFN风险的妊娠中具有显着优势。

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