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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Inducible protective processes in animal systems XV: Hyperthermia enhances the Ethyl methanesulfonate induced adaptive response in meiotic cells of grasshopper Poecilocerus pictus
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Inducible protective processes in animal systems XV: Hyperthermia enhances the Ethyl methanesulfonate induced adaptive response in meiotic cells of grasshopper Poecilocerus pictus

机译：动物系统中的诱导性保护过程十五：热疗增强了甲磺酸乙酯诱导的蚱hopper减数分裂细胞的适应性反应 Poecilocerus pictus

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Purpose To understand the role of hyperthermia in adaptive response, Ethyl methanesulfonate (EMS) an anticarcinogenic agent, adapted meiotic cells of Poecilocerus pictus was used. Materials and methods Based on the pilot toxicity study, the effective higher temperatures of 40 °C and 45 °C for 15 or 30 min were chosen. P. pictus were treated with conditioning (L) or challenging (H) doses of EMS and 2 h time lag (TL) between these doses (L-2 h-H) was employed. Different treatment schedules were used to analyze the influence of hyperthermia on EMS induced adaptive response namely (i) pre treatment; (ii) inter treatment; (iii) post treatment and (iv) cross adaptation. After each treatment schedule, animals were sacrificed at 12, 24, 36 and 48 h recovery times, testes were processed for meiotic chromosome preparations and anomalies were analyzed. Results The frequencies of anomalies induced by both conditioning and challenging doses of EMS were significantly higher ( p 0.05) compared to those of the control and hyperthermia groups. The combined treatments resulted in 44–50% reduction compared to additive effect of EMS. The pre, inter, post and cross adaptation treatments with hyperthermia significantly reduced the frequencies of chromosomal anomalies compared to the challenge and combined treatments with EMS at all recovery times ( p 0.05) tested. Conclusion There is a protection against EMS induced anomalies by hyperthermia in in vivo P. pictus . As far as our knowledge is concerned, this is the first report to demonstrate that hyperthermia enhances the EMS induced adaptive response in in vivo meiotic cells.
机译：目的为了了解热疗在适应性反应中的作用，使用了甲磺酸乙酯（EMS）作为抗癌剂，使用了适应性的Poecilocerus pictus减数分裂细胞。材料和方法根据初步毒性研究，选择40℃和45℃的有效高温15或30分钟。用条件适应性（L）或挑战性（H）剂量的EMS处理小白菜（P. pictus），并在这些剂量之间（L-2 h-H）使用2小时的时间间隔（TL）。使用不同的治疗方案来分析热疗对EMS诱导的适应性反应的影响，即（i）预处理; （ii）间治疗; （iii）后处理和（iv）交叉适应。在每个治疗方案之后，在恢复的第12、24、36和48小时处死动物，处理睾丸以进行减数分裂染色体制备并分析异常情况。结果与对照组和热疗组相比，调节和挑战剂量的EMS引起的异常频率明显更高（p <0.05）。与EMS的累加作用相比，联合治疗可减少44-50％。在所有测试的恢复时间内，与挑战和联合EMS联合治疗相比，热疗的适应前，间，后和交叉适应治疗均显着降低了染色体异常发生的频率（p <0.05）。结论体内高温对EMS引起的高温异常具有一定的保护作用。就我们所知，这是第一份证明热疗可增强EMS诱导的体内减数分裂细胞适应性反应的报告。

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