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首页> 外文期刊>Iranian Journal of Reproductive Medicine >THE EFFECTS OF VITRIFICATION ON SPINDLE APPARATUS OF IN VITRO MATURED GERMINAL VESICLE IN MICE
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THE EFFECTS OF VITRIFICATION ON SPINDLE APPARATUS OF IN VITRO MATURED GERMINAL VESICLE IN MICE

机译：玻璃化对小鼠体外成熟的胚泡主轴装置的影响

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Background: Routine oocytes cryopreservation remained an elusive technique in the wide ranges of available assisted reproductive technologies. The microtubules of oocytes are vulnerable to cryoprotectants and thermal change during cryopreservation.Objective: The effects of a vitrification protocol were investigated on the spindle and chromosome configurations of mice oocytes cryopreserved at the germinal vesicle stage.Materials and Methods: Germinal vesicle with cumulus cells were transferred to vitrification solution which was composed of 30% (v/v) ethylene glycol, 18% Ficoll-70 and 0.3 M Sucrose either by single step or in step-wise way. Following vitrification and in vitro maturation (MII), the matured oocytes were immonostained for meiotic spindles and chromosomes, before visualization using fluorescent microscopy. Results: A statistically significant increase was observed in the survival and maturation rate in step-wise vitrification (88.96% and 71.23% respectively) compared to single step vitrification (70.6% and 62.42% respectively) (p0.05). Normal spindle morphology after vitrification-thawing in step-wise vitrification group (77.26%) was higher than single step vitrification group (64.24%) but lower than control group (94.75%) (p0.05).Conclusion: The results suggest that vitrification with step-wise procedure on mice germinal vesicle oocytes has positive effects on survival and maturation rate and normal spindle configuration compare with single step vitrification procedure.
机译：背景：在许多可用的辅助生殖技术中，常规卵母细胞冷冻保存仍是一项难以捉摸的技术。目的：研究玻璃化方案对生发囊泡期冷冻保存的小鼠卵母细胞纺锤体和染色体构型的影响。通过一步法或分步法将其转移到由30％（v / v）乙二醇，18％Ficoll-70和0.3 M蔗糖组成的玻璃化溶液中。玻璃化和体外成熟（MII）之后，将成熟的卵母细胞对减数分裂纺锤体和染色体进行单染色，然后使用荧光显微镜观察。结果：与单步玻璃化（分别为70.6％和62.42％）相比，逐步玻璃化的存活率和成熟率（分别为88.96％和71.23％）具有统计学意义的提高（p <0.05）。逐步玻璃化组融化后纺锤体形态正常（77.26％）高于单步玻璃化组（64.24％），但低于对照组（94.75％）（p <0.05）。结论：玻璃化与单步玻璃化法相比，分步法对小鼠生发囊泡卵母细胞对存活和成熟率及正常纺锤体构型具有积极影响。

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