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首页> 外文期刊>Iranian Journal of Reproductive Medicine >CORRELATION BETWEEN SPERM PARAMETERS AND SPERM DNA FRAGMENTATION IN FERTILE AND INFERTILE MEN
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CORRELATION BETWEEN SPERM PARAMETERS AND SPERM DNA FRAGMENTATION IN FERTILE AND INFERTILE MEN

机译：男人和女人的精子参数与精子DNA片段的相关性

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Background: It is established that sperm DNA integrity is essential in fertilization and normal embryo and fetal development. Routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm DNA. Therefore, the evaluation of sperm DNA integrity, in addition to routine sperm parameters, could add further information on the quality of spermatozoa and reproductive potential of males.Objective: The objective of this study was to determine the levels of sperm DNA damage in fertile and infertile males and its correlation with semen parameters.Materials and Methods: Semen samples were collected from 45 infertile men selected from couples attending the infertility clinic with a history of infertility of a‰￥1 years and 75 healthy volunteers of proven fertility (initiated a successful pregnancy) served as the control group. After routine sperm analysis, DNA damage was determined using single cell gel electrophoresis (comet) assay method.Results: The mean of DNA damage (comet value) in the sperms of infertile males was significantly higher than that of fertile males (12.9?±7.59 vs. 48.77?±24.42, p0.001). A significant negative correlation was observed between DNA damage and sperm motility in fertile group (p0.02, R=-0.263). In infertile males, significant negative correlations were observed between DNA damage with sperm motility (p0.002, R=-0.45) and morphology (p0.03, R=-0.317). There was no significant correlation between sperm concentration and sperm DNA damage in both groups.Conclusion: These results indicate that sperm DNA damages in infertile males is significantly higher than fertile males and sperms with abnormal morphology and low levels of motility has more abnormal DNA damages than motile and normal sperms.
机译：背景：已确定精子DNA完整性对于受精，正常胚胎和胎儿发育至关重要。常规精液分析可对精子的功能能力进行大致评估，但并不总能反映出精子DNA的质量。因此，除了常规的精子参数外，对精子DNA完整性的评估还可以提供有关精子质量和男性生殖潜能的进一步信息。目的：本研究的目的是确定可育和精子中精子DNA损伤的水平。材料和方法：收集45例不育男性夫妇的精液样本，这些男性在不育诊所就诊，这些夫妇有不育历史，≥1年，不育者年龄≥1年，经证实具有生育能力的75名健康志愿者（成功启动怀孕）作为对照组。常规精子分析后，采用单细胞凝胶电泳（彗星）测定法测定DNA损伤。结果：不育男性精子中DNA损伤（彗星值）的平均值显着高于可育男性（12.9±7.59）。与48.77？±24.42，p <0.001）。在可育组中，DNA损伤与精子活力之间存在显着的负相关（p <0.02，R = -0.263）。在不育男性中，观察到DNA损伤与精子活力（p <0.002，R = -0.45）和形态学（p <0.03，R = -0.317）之间存在显着的负相关。两组的精子浓度与精子DNA损伤之间无显着相关性。结论：这些结果表明，不育男性的精子DNA损伤显着高于可育男性，形态异常，运动水平低的精子的异常DNA损伤要多于精子。运动和正常的精子。

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