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首页> 外文期刊>Iranian Journal of Reproductive Medicine >DEVELOPMENTAL CONSEQUENCES OF MOUSE CRYOTOP-VITRIFIED OOCYTE AND EMBRYO USING LOW CONCENTRATED CRYOPROTECTANTS
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DEVELOPMENTAL CONSEQUENCES OF MOUSE CRYOTOP-VITRIFIED OOCYTE AND EMBRYO USING LOW CONCENTRATED CRYOPROTECTANTS

机译：低浓度冷冻保护剂对小鼠冷冻卵囊卵和胚胎发育的影响

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Background: The risk of multiple pregnancies, often present in programs of In Vitro Fertilization (IVF), is an important force for embryo cryopreservation. On the other hand, ethical restriction and assurance of potential fertility following chemo/radio therapy has led scientists to focus on female gamete preservation. Objective: Optimizing vitrification protocol by using less concentrated cryoprotectants (CPAs) in order to decrease CPAs toxicity.  Materials and Methods: Mouse Metaphase-II (M-II) oocytes and four cell-stage embryos were collected. Oocytes Survival, Fertilization and Developmental Rates (SRs, FRs, DRs) were recorded after cryotop-vitrification/warming. As well as comparing fresh oocytes and embryos, the data obtained from experimental groups (exp.) applying 1.25, 1.0, 0.75 molar (M) CPAs were analyzed in comparison to those of adopting 1.5 M CPAs [largely-used concentration of Ethylen Glycol (EG) and Dimethyl-sulphoxide (DMSO)].Results: The data of oocytes exposed to 1.25 M concentrated CPAs were in consistency with those exposed to 1.5 M and control group in terms of SR, FR and DR. As less concentration was applied, the more decreased SRs, FRs and DRs were obtained from other experimental groups. The results of embryos which were exposed to 1.25 M and 1.0 M were close to those vitrified with 1.5 M and fresh embryos. The results of 0.75 M concentrated CPAs solutions were significantly lower than those of control, 1.5 M and 1.0 M treated groups.Conclusion: CPAs limited reduction to 1.25 M and 1.0 M instead of using 1.5 M, for oocyte and embryo cryotop-vitrification procedure may be a slight adjustment.
机译：背景：在体外受精（IVF）程序中经常出现多次怀孕的风险，是胚胎冷冻保存的重要力量。另一方面，化学/放射疗法后的伦理限制和对潜在生育力的保证已导致科学家将重点放在女性配子的保存上。目的：通过使用浓度较低的冷冻保护剂（CPA）优化玻璃化方案，以降低CPA的毒性。材料和方法：收集小鼠中期II（M-II）卵母细胞和四个细胞阶段的胚胎。冰冻玻璃化/加温后，记录卵母细胞的存活，受精和发育率（SR，FR，DR）。除了比较新鲜的卵母细胞和胚胎外，还分析了从实验组（实验组）获得的1.25、1.0、0.75摩尔（M）CPA的数据与采用1.5 M CPA的数据的比较。结果：暴露于1.25 M浓缩CPA的卵母细胞的数据与SR，FR和DR方面的数据与暴露于1.5 M的CPA和对照组的卵母细胞的数据一致。随着浓度的降低，从其他实验组获得的SR，FR和DR减少得更多。暴露于1.25 M和1.0 M的胚胎的结果接近于用1.5 M和新鲜胚胎进行玻璃化的结果。 0.75 M浓缩CPAs溶液的结果明显低于对照组，1.5 M和1.0 M处理组。稍作调整。

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