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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Clinical Utility of promoter methylation of the tumor suppressor genes DKK3, and RASSF1A in breast cancer patients
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Clinical Utility of promoter methylation of the tumor suppressor genes DKK3, and RASSF1A in breast cancer patients

机译：抑癌基因DKK3和RASSF1A的启动子甲基化在乳腺癌患者中的临床应用

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摘要

Background DNA methylation is the commonest known epigenetic change that results in silencing of tumor suppressor genes. Promoter methylation of tumor suppressor genes has the potential for early detection of breast cancer. Aim Aim is to examine the potential usefulness of blood based methylation specific polymerase chain reaction (MSP) of methylated DKK3 and RASSF1A genes in early detection of breast cancer. Method Methylation status of DKK3 and RASSF1 was investigated in forty breast cancer patients, twenty fibroadenoma patients and twenty healthy ladies as control group using MSP. Results Methylation of DKK3 promoter was found in 22.5% of breast cancer patients, while DKK3 methylation was absent in both fibroadenoma patients and control group. Similarly, methylation of RASSF1 promoter was found in 17.5% of breast cancer patients and in none of fibroadenoma and control group. Conclusion Promoter methylation of DKK3 and RASSF1 was found in breast cancer patients while absent in control group suggesting that tumorspecific methylation of the two genes (DKK3 and RASSF1A) might be a valuable biomarker for the early detection of breast cancer.
机译：背景DNA甲基化是导致肿瘤抑制基因沉默的最常见的表观遗传变化。肿瘤抑制基因的启动子甲基化具有早期发现乳腺癌的潜力。目的目的是研究基于甲基化的DKK3和RASSF1A基因的基于血液的甲基化特异性聚合酶链反应（MSP）在乳腺癌的早期检测中的潜在用途。方法采用MSP法对40例乳腺癌患者，20例纤维腺瘤患者和20例健康女性作为对照组，观察DKK3和RASSF1的甲基化状态。结果乳腺癌患者中22.5％发现DKK3启动子甲基化，而纤维腺瘤患者和对照组均未发现DKK3启动子甲基化。同样，在17.5％的乳腺癌患者中发现RASSF1启动子甲基化，而在纤维腺瘤和对照组中均未发现。结论在乳腺癌患者中发现DKK3和RASSF1的启动子甲基化，而在对照组中则没有，提示这两个基因（DKK3和RASSF1A）的肿瘤特异性甲基化可能是早期发现乳腺癌的有价值的生物标志物。

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