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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse
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Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse

机译：生化参数对EMS和MMS处理的差异反应及其对糖尿病小鼠染色体的剂量效应关系

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Aim To study the effect of alkylating agents such as EMS and MMS on chromosomes and biochemical parameters in induced diabetic mouse. Methods Chromosome preparations from bone marrow was made using the method of Evans et al. (1964) and biochemical estimations from the liver was done by the method of Sinha (1972) for catalase, Van der Vies (1954) for glycogen and Uchiyama and Mihara (1978) for MDA. Results The study has revealed that EMS and MMS induced a dose dependent increase in chromosomal aberrations of chromatid type in the diabetic mouse. Nonetheless, it is interesting to note that, there is significant reduction in the frequency of chromosomal aberrations in diabetic compared to non diabetic mice at all tested doses of EMS or MMS and at different recovery times [RTs]. On the other hand biochemical parameters showed a variable degree of reactivity: (1) catalase activity was significantly elevated in non diabetics whereas in diabetics it is significantly decreased with increasing concentrations of EMS. Contrary to this, the catalase activity in the case of MMS treatment is significantly reduced in non diabetics compared to diabetic mice. (2) However glycogen level is significantly reduced in both the diabetic and non diabetic with increasing concentrations of EMS or MMS, but MDA levels were significantly increased. Conclusion (1) Even though alkylating agents induce chromosomal aberrations in diabetic mice, MMS, a methylating agent is a more potent inducer of chromatid type of aberrations than EMS, an ethylating agent. (2) Diabetic mouse is more resistant than the non diabetic to alkylating agents and (3) the tested agents altered the analyzed biochemical parameters.
机译：目的研究EMS和MMS等烷基化剂对糖尿病小鼠染色体和生化指标的影响。方法使用Evans等人的方法从骨髓中制备染色体。 （1964）和肝脏的生化评估是通过Sinha（1972）的过氧化氢酶，Van der Vies（1954）的糖原和Uchiyama和Mihara（1978）的MDA方法进行的。结果研究表明，EMS和MMS可以诱导糖尿病小鼠中染色单体型染色体畸变的剂量依赖性增加。但是，有趣的是，在所有测试剂量的EMS或MMS以及不同的恢复时间[RTs]上，与非糖尿病小鼠相比，糖尿病患者的染色体畸变频率显着降低。另一方面，生化参数显示出不同程度的反应性：（1）过氧化氢酶活性在非糖尿病患者中显着升高，而在糖尿病患者中，随着EMS浓度的增加，过氧化氢酶活性显着降低。与此相反，与糖尿病小鼠相比，非糖尿病患者在MMS治疗情况下的过氧化氢酶活性显着降低。 （2）然而，随着EMS或MMS浓度的增加，糖尿病患者和非糖尿病患者的糖原水平均显着降低，但MDA水平显着升高。结论（1）尽管烷基化剂会引起糖尿病小鼠染色体畸变，但是MMS（一种甲基化剂）比EMS（一种乙基化剂）对染色单体型畸变的诱导作用更强。 （2）糖尿病小鼠比非糖尿病小鼠对烷基化剂的抵抗力更强，并且（3）被测药物改变了所分析的生化参数。

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