...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Ankaferd Blood Stopper induces apoptosis and regulates PAR1 and EPCR expression in human leukemia cells
【24h】

Ankaferd Blood Stopper induces apoptosis and regulates PAR1 and EPCR expression in human leukemia cells

机译：Ankaferd止血剂诱导人白血病细胞凋亡并调节PAR1和EPCR表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Ankaferd Blood Stopper (ABS) is a preparation of plant extracts originally used as a hemostatic agent. It has pleiotropic effects in many cellular processes such as cell cycle regulation, apoptosis, angiogenesis, signal transduction, inflammation, immunologic processes and metabolic pathways as well as hemostatic activity. This unique preparation has been widely investigated for its properties. However there are no studies investigating its action on leukemic cells. Aim Aim of the study was to examine the ABS action on PAR1 and EPCR in leukemia cells. However, during the experiments, we observed the apoptotic effect of ABS on leukemic cells, particularly Jurkat cells. As a result the mechanism of apoptosis induced by ABS treatment was also explored in the study. Material and method Two leukemia cell lines, K-562 and Jurkat, were utilized for the study. Expression analyses of PAR1, EPCR and p21 upon ABS treatment were performed by quantitative real time PCR. Annexin V method was used for apoptosis detection. Results Our results demonstrated that ABS alters PAR1 and EPCR expression in K-562 and Jurkat cells in a time and dose dependent manner. Additionally it was found that ABS treatment induces apoptosis in leukemia cells. Possible involvement of PAR1 and p21 in this apoptotic process was observed in Jurkat cells. Conclusion This study concludes that depending on the concentration and duration of the application, ABS causes apoptosis by regulating PAR1 and p53-independent p21 involvement in apoptosis stimulation in leukemia cells. The composition of ABS plant extracts might be responsible from the apoptotic effect that was observed. We think that our results could contribute to the development of new treatment for leukemia therapy.
机译：背景Ankaferd止血剂（ABS）是最初用作止血剂的植物提取物的制剂。它在许多细胞过程中具有多效作用，例如细胞周期调节，凋亡，血管生成，信号转导，炎症，免疫过程和代谢途径以及止血活性。这种独特的制剂已被广泛研究其性能。但是，尚无研究其对白血病细胞作用的研究。目的本研究的目的是检查ABS对白血病细胞中PAR1和EPCR的作用。但是，在实验过程中，我们观察到ABS对白血病细胞，特别是Jurkat细胞的凋亡作用。结果，在该研究中还探索了由ABS处理诱导的细胞凋亡的机制。材料和方法利用两种白血病细胞系K-562和Jurkat进行研究。通过定量实时PCR进行ABS处理后PAR1，EPCR和p21的表达分析。 Annexin V方法用于细胞凋亡检测。结果我们的结果表明，ABS以时间和剂量依赖性方式改变K-562和Jurkat细胞中的PAR1和EPCR表达。另外，发现ABS治疗诱导白血病细胞凋亡。在Jurkat细胞中观察到PAR1和p21可能参与了这一凋亡过程。结论：本研究的结论是，根据应用的浓度和持续时间，ABS通过调节PAR1和p53独立的p21参与白血病细胞凋亡刺激而引起凋亡。 ABS植物提取物的成分可能是由观察到的细胞凋亡作用引起的。我们认为我们的结果可能有助于开发白血病新疗法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号