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首页> 外文期刊>Electronic Journal of Biology >Purpureocillium lilacinum Strain BP13 Produces Flavoglaucin
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Purpureocillium lilacinum Strain BP13 Produces Flavoglaucin

机译：紫丁香紫丁香菌株BP13产生黄霉素。

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Liquid chromatograph/quardapole-time of flight/mass spectrometry (LC/Q-TOF/MS) is a modern analytical approach in the rapid screening of crude extracts of biological sample for the characterization of secondary metabolites. LC/Q-TOF/MS offers new possibilities for studying fungal metabolites in its crude form, as it requires no sample cleaning. Furthermore, targeted analysis is also needless as the instrument can provide accurate mass measurement of molecular ions of any components detected in an LC run, and these can be identified by searching a database of exact masses of relevant secondary metabolites, which is linked to the analytical system. In the present study, Purpureocillium lilacinum strain BP13 (a novel isolate from this laboratory) was grown in potato dextrose medium for 14 days and the supernatant was extracted in ethyl acetate and subjected to LC-Q-TOF-MS analysis. The peak at 6.03 min had a mass of m/z 305.2118, exactly corresponding to flavoglaucin with chemical formulae C19H28O3, based on the available fungal database search. Literature shows that flavoglaucin is an excellent antioxidant and a synergizing agent, and also shown to have antibacterial and anticancer properties. This is the first report on the production of flavoglaucin by P. lilacinum.
机译：液相色谱/四极杆飞行时间/质谱（LC / Q-TOF / MS）是一种现代分析方法，可快速筛选生物样品的粗提物以鉴定次级代谢物。 LC / Q-TOF / MS由于无需清洗样品，因此为研究粗制真菌代谢产物提供了新的可能性。此外，也无需进行目标分析，因为该仪器可以对LC运行中检测到的任何成分的分子离子进行准确的质量测量，并且可以通过搜索相关次级代谢物精确质量的数据库来识别这些离子，该数据库与分析方法相关联。系统。在本研究中，紫丁香purpureocillium laclacum菌株BP13（该实验室的新型分离株）在马铃薯葡萄糖培养基中生长14天，上清液在乙酸乙酯中萃取并进行LC-Q-TOF-MS分析。根据可用的真菌数据库搜索，在6.03分钟时的峰质量为m / z 305.2118，与化学式为C19H28O3的黄酮青霉素完全对应。文献表明黄酮青霉素是极好的抗氧化剂和增效剂，并且还显示出具有抗菌和抗癌的特性。这是关于紫丁香假单胞菌生产黄酮青霉素的首次报道。

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