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首页> 外文期刊>Electronic Journal of Biotechnology >Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars
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Development, characterization and use of genomic SSR markers for assessment of genetic diversity in some Saudi date palm (Phoenix dactylifera L.) cultivars

机译：开发，表征和使用基因组SSR标记评估某些沙特枣椰子（Phoenix dactylifera L.）品种的遗传多样性

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Background: The present study was undertaken towards the development of SSR markers and assessing genetic relationships among 32 date palm (Phoenix dactylifera L.) representing common cultivars grown in different geographical regions in Saudi Arabia. Results: Nintythree novel simple sequence repeat markers were developed and screened for their ability to detect polymorphism in date palm. Around 71% of genomic SSRs were dinucleotide, 25% tri, 3% tetra and 1% penta nucleotide motives. Twenty-two primers generated a total of 91 alleles with a mean of 4.14 alleles per locus and 100% polymorphism percentage. A 0.595 average polymorphic information content and 0.662 primer discrimination power values were recorded. The expected and observed heterozygosities were 0.676 and 0.763 respectively. Pair-wise similarity values ranged from 0.06 to 0.89 and the overall cultivars averaged of 0.41. The UPGMA cluster analysis recovered by principal coordinate analysis illustrated that cultivars trend to group according to their class of maturity, region of cultivation, and fruit color. Analysis of molecular variations (AMOVA) revealed that genetic variation among and within cultivars were 27% and 73%, respectively according to geographical distribution of cultivars.Conclusions: The developed microsatellite markers are additional values to date palm characterization tools can be used by researchers in population genetics, cultivars identification as well as genetic resources exploration and management. The tested cultivars exhibited a significant amount of genetic diversity and could be suitable for successful breeding program. Genomic sequences generated from this study are available at National Center for Biotechnology Information (NCBI), Sequence Read Archive (Accession numbers. LIBGSS_039019).
机译：背景：本研究旨在开发SSR标记并评估32种枣椰子（Phoenix dactylifera L.）之间的遗传关系，这些枣椰子代表了在沙特阿拉伯不同地理区域生长的普通品种。结果：开发了九十三种新颖的简单序列重复标记，并筛选了它们检测枣椰子中多态性的能力。大约71％的基因组SSR是二核苷酸，25％的三核苷酸，3％的四核苷酸和1％的五核苷酸动机。 22条引物共产生91个等位基因，每个基因座平均为4.14个等位基因，且多态性百分比为100％。记录0.595平均多态信息含量和0.662引物鉴别能力值。预期和观察到的杂合度分别为0.676和0.763。成对相似度值范围在0.06到0.89之间，整体品种平均为0.41。通过主坐标分析得到的UPGMA聚类分析表明，根据品种的成熟程度，种植区域和果实颜色，其趋向于分组。分子变异分析（AMOVA）显示，根据品种的地理分布，品种之间和内部的遗传变异分别为27％和73％。结论：开发的微卫星标记是迄今为止棕榈树表征工具可用于研究人员的附加值。种群遗传学，品种鉴定以及遗传资源的探索和管理。被测品种显示出大量的遗传多样性，可能适合成功的育种程序。这项研究产生的基因组序列可在国家生物技术信息中心（NCBI）的序列读取档案库中获得（登录号：LIBGSS_039019）。

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