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首页> 外文期刊>Einstein (So Paulo) >Extracellular adenosine 5a??-triphosphate concentrations changes in rat spinal cord associated with the activation of urinary bladder afferents. A microdialysis study
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Extracellular adenosine 5a??-triphosphate concentrations changes in rat spinal cord associated with the activation of urinary bladder afferents. A microdialysis study

机译：大鼠脊髓中胞外腺苷5aβ-三磷酸的浓度变化与膀胱传入神经的激活有关。微透析研究

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Objective To determine adenosine 5a??-triphosphate levels in the interstice of spinal cord L 6 -S 1 segment, under basal conditions or during mechanical and chemical activation of urinary bladder afferents. Methods A microdialysis probe was transversally implanted in the dorsal half of spinal cord L 6 -S 1 segment in female rats. Microdialysate was collected at 15 minutes intervals during 135 minutes, in anesthetized animals. Adenosine 5a??-triphosphate concentrations were determined with a bioluminescent assay. In one group of animals (n=7) microdialysate samples were obtained with an empty bladder during a 10-minutes bladder distension to 20 or 40cmH 2 O with either saline, saline with acetic acid or saline with capsaicin. In another group of animals (n=6) bladder distention was performed and the microdialysis solution contained the ectonucleotidase inhibitor ARL 67156. Results Basal extracellular adenosine triphosphate levels were 110.9?±35.34fmol/15 minutes, (mean?±SEM, n=13), and bladder distention was associated with a significant increase in adenosine 5a??-triphosphate levels which was not observed after bladder distention with saline solution containing capsaicin (10?μM). Microdialysis with solution containing ARL 67156 (1mM) was associated with significantly higher extracellular adenosine 5a??-triphosphate levels and no further increase in adenosine 5a??-triphosphate was observed during bladder distension. Conclusion Adenosine 5a??-triphosphate was present in the interstice of L 6 -S 1 spinal cord segments, was degraded by ectonucleotidase, and its concentration increased following the activation of bladder mechanosensitive but not of the chemosensitive afferents fibers. Adenosine 5a??-triphosphate may originate either from the central endings of bladder mechanosensitive primary afferent neurons, or most likely from intrinsic spinal neurons, or glial cells and its release appears to be modulated by capsaicin activated bladder primary afferent or by adenosine 5a??-triphosphate itself.
机译：目的测定在基础条件下或在机械和化学激活膀胱传入神经的过程中，脊髓L 6 -S 1段间隙中腺苷5aβ-三磷酸水平。方法将微透析探针横向植入雌性大鼠脊髓L 6 -S 1的背半段。在135分钟内以15分钟为间隔在麻醉动物中收集微量透析液。用生物发光测定法测定5aβ-三磷酸腺苷的浓度。在一组动物（n = 7）中，用空的膀胱在10分钟内用盐水，含乙酸的盐水或含辣椒素的盐水膨胀至20或40cmH 2 O，获得微量透析液样品。在另一组动物中（n = 6）进行了膀胱扩张术，微透析溶液中含有外核苷酸酶抑制剂ARL67156。结果基底细胞三磷酸腺苷水平为110.9？±35.34fmol / 15分钟，（平均值？±SEM，n = 13 ），并且膀胱扩张与腺苷5aβ-三磷酸水平的显着增加有关，而在含有辣椒素（10？μM）的盐水溶液使膀胱扩张后没有观察到。用含有ARL 67156（1mM）的溶液进行微透析与明显升高的细胞外腺苷5aβ-三磷酸水平有关，在膀胱膨胀期间未观察到腺苷5aβ-三磷酸进一步增加。结论L 6 -S 1脊髓节段中存在腺苷5aβ-三磷酸，被外切核苷酸酶降解，并且其浓度随着膀胱机械敏感而不是化学敏感传入纤维的激活而增加。腺苷5aβ-三磷酸可能起源于膀胱机械敏感的初级传入神经元的中央末端，或最有可能源自内在脊神经元或神经胶质细胞，其释放似乎受辣椒素激活的膀胱初级传入神经元或腺苷5aβ的调节。 -三磷酸本身。

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