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首页> 外文期刊>Einstein (So Paulo) >Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line
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Intracellular labeling and quantification process by magnetic resonance imaging using iron oxide magnetic nanoparticles in rat C6 glioma cell line

机译：氧化铁磁性纳米粒子在大鼠C6胶质瘤细胞系中的磁共振成像细胞内标记和定量过程

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OBJECTIVE: To assess intracellular labeling and quantification by magnetic resonance imaging using iron oxide magnetic nanoparticles coated with biocompatible materials in rat C6 glioma cells in vitro. These methods will provide direction for future trials of tumor induction in vivo as well as possible magnetic hyperthermia applications. METHODS: Aminosilane, dextran, polyvinyl alcohol, and starch-coated magnetic nanoparticles were used in the qualitative assessment of C6 cell labeling via light microscopy. The influence of the transfection agent poly-L-lysine on cellular uptake was examined. The quantification process was performed by relaxometry analysis in T1 and T2weighted phantom images. RESULTS: Light microscopy revealed that the aminosilane-coated magnetic nanoparticles alone or complexed with poly-L-lysine showed higher cellular uptake than did the uncoated magnetic particles. The relaxivities of the aminosilane-coated magnetic nanoparticles with a hydrodynamic diameter of 50nm to a 3-T field were r1=(6.1±0.3)×10-5 ms-1mL/μg, r2=(5.3±0.1)× 10-4 ms-1mL/μg, with a ratio of r2 / r1 ≅ 9. The iron uptake in the cells was calculated by analyzing the relaxation rates (R1 and R2) using a mathematical relationship. CONCLUSIONS: C6 glioma cells have a high uptake efficiency for aminosilane-coated magnetic nanoparticles complexed with the transfection agent poly-L-lysine. The large ratio r2 / r1 ≅ 9 indicates that these magnetic nanoparticles are ideal for quantification by magnetic resonance imaging with T2-weighted imaging techniques.
机译：目的：通过使用生物相容性材料包被的氧化铁磁性纳米粒子在大鼠C6神经胶质瘤细胞中进行磁共振成像来评估细胞内标记和定量。这些方法将为体内肿瘤诱导的未来试验以及可能的磁热疗应用提供指导。方法：使用氨基硅烷，葡聚糖，聚乙烯醇和淀粉包覆的磁性纳米粒子通过光学显微镜对C6细胞标记进行定性评估。检查了转染剂聚-L-赖氨酸对细胞摄取的影响。通过弛豫分析在T1和T2加权幻像中执行量化过程。结果：光学显微镜显示，单独或与聚-L-赖氨酸复合的氨基硅烷包覆的磁性纳米颗粒比未包覆的磁性颗粒具有更高的细胞摄取。流体动力学直径为50nm的氨基硅烷包覆的磁性纳米粒子对3-T场的弛豫度为r1 =（6.1±0.3）×10-5 ms-1mL /μg，r2 =（5.3±0.1）×10-4 ms-1mL /μg，比率为r2 / r1 =9。通过使用数学关系式分析弛豫率（R1和R2）来计算细胞中的铁吸收。结论：C6神经胶质瘤细胞对氨基硅烷包覆的磁性纳米粒子与转染剂聚-L-赖氨酸复合后具有很高的摄取效率。 r2 / r1 = 9的大比率表明，这些磁性纳米粒子非常适合通过T2加权成像技术进行磁共振成像定量。

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