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首页> 外文期刊>Endocrine journal >Effects of Dehydroepiandrosterone on Gluconeogenic Enzymes and Glucose Uptake in Human Hepatoma Cell Line, HepG2
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Effects of Dehydroepiandrosterone on Gluconeogenic Enzymes and Glucose Uptake in Human Hepatoma Cell Line, HepG2

机译：脱氢表雄酮对人肝癌细胞HepG2糖原生成酶和葡萄糖摄取的影响

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References(24) Cited-By(21) Dehydroepiandrosterone (DHEA), the most abundant human adrenal steroid, improves insulin sensitivity and obesity in human and model animals. In a previous study, we reported that orally administered DHEA suppresses the elevated activities of hepatic gluconeogenic enzymes like glucose-6-phosphatase (G6Pase) in C57BL/KsJ-db/db mice (Aoki K, Saito T, Satoh S, Mukasa K, Kaneshiro M, Kawasaki S, Okamura A, Sekihara H (1999) Diabetes 48: 1579–1585). However, the molecular mechanisms by which DHEA ameliorates insulin resistance are not clearly understood. In the present study, we cultured the human hepatoma cell line HepG2 with DHEA and measured the enzyme activity and protein expression of G6Pase to investigate the direct effect of DHEA on glucose metabolism in hepatocytes. DHEA significantly suppressed both the activity and protein expression of G6Pase. Moreover, DHEA decreased the gene expression of G6Pase and phosphoenolpyruvate carboxykinase, both of which were maximal at 1 μM DHEA, whereas the mRNA level of glucose-6-phosphate translocase was unchanged. Furthermore, DHEA enhanced 2-deoxyglucose uptake, although its effect was much smaller than that of insulin. These results suggest that DHEA may act at multiple steps in the regulation of glucose metabolism in the liver.
机译：参考文献（24）被引用的By（21）脱氢表雄酮（DHEA）是最丰富的人类肾上腺类固醇，可改善人类和模型动物的胰岛素敏感性和肥胖症。在先前的研究中，我们报道了口服DHEA可以抑制C57BL / KsJ-db / db小鼠（Aoki K，Saito T，Satoh S，Mukasa K，金城四郎，川崎S，冈村A，关原H（1999）糖尿病48：1579-1585）。但是，尚不清楚DHEA改善胰岛素抵抗的分子机制。在本研究中，我们用DHEA培养人肝癌细胞系HepG2，并测量G6Pase的酶活性和蛋白表达，以研究DHEA对肝细胞葡萄糖代谢的直接作用。 DHEA显着抑制G6Pase的活性和蛋白质表达。此外，DHEA降低了G6Pase和磷酸烯醇丙酮酸羧化激酶的基因表达，两者在1μMDHEA时最大，而6磷酸葡萄糖转运蛋白的mRNA水平没有变化。此外，DHEA增强了2-脱氧葡萄糖的摄取，尽管它的作用远小于胰岛素。这些结果表明，DHEA可能在肝脏葡萄糖代谢的调节中起多个作用。

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