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首页> 外文期刊>Environment and Natural Resources Research >Modulation of Mutagen-Induced Genotoxicity by two Lesotho Medicinal Plants in Allium cepa L
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Modulation of Mutagen-Induced Genotoxicity by two Lesotho Medicinal Plants in Allium cepa L

机译：洋葱中两种莱索托药用植物对诱变的遗传毒性的调控

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摘要

Methanolic extracts (mg mL-1) of Dicoma anomala (0.0625, 0.125, 0.25), Chenopodium album (0.078, 0.156, 0.313), used in traditional medicine in Lesotho, and Camellia sinensis (0.1094, 0.2085, 0.44) were assessed for cytotoxicity, genotoxicity and modulation of Cyclophosphamide (CP 1.25 mg mL-1) - and EMS (0.25 mg mL-1)-induced genotoxicity using the Allium cepa assay following 24 hours treatment. Cytotoxicity was measured by the mitotic index (MI). Genotoxicity (GT) was expressed as the number of aberrant mitotic cells per 100 mitotic cells. The MI and GT of test groups (triplicates), were compared with the negative (water) control group using t-test. Modulatory effect (ME) was calculated as, ME(%) = (1-(A-B)/(A-C) × 100) -1. A positive ME indicated an increase (synergism or potentiation) while a negative ME indicated a reduction (anti-genotoxicity) of mutagen-induced genotoxicity. The concentrations of D. anomala extract were cytotoxic and genotoxic. Mixtures of CP or EMS with the lower concentrations of D. anomala extract were more genotoxic than CP (250.50% increase) or EMS (149.74 and 157.37 % increase) or extracts alone. Only 0.313 mg mL-1 of C. album extract was cytotoxic but none of the three concentrations was genotoxic. Mixtures of CP with extracts of C.album were cytotoxic. CP-induced genotoxicity was reduced (55.18, 68.36, 57.40 %) and EMS-induced genotoxicity was also reduced by low concentrations (50.72, 61.13 %) of C.album extract. However, 0.313 mg mL-1 C. album extract increased (236.75%) EMS-induced genotoxicity. C. sinensis extracts and their mixtures with CP were not cytotoxic or genotoxic. CP-induced genotoxicity was reduced (63.61, 66.62, 78.64 %) but EMS-induced genotoxicity was increased (124.97, 4.48, 110.52 %) by C. sinensis extract.
机译：评估了莱索托传统药物中使用的Dicoma anomala（0.0625，0.125，0.25），白藜（0.078，0.156，0.313）的甲醇提取物（mg mL-1）和山茶（0.1094，0.2085，0.44）的细胞毒性处理24小时后，使用葱属cepa测定，环磷酰胺（CP 1.25 mg mL-1）和EMS（0.25 mg mL-1）引起的遗传毒性和调节。通过有丝分裂指数（MI）来测量细胞毒性。遗传毒性（GT）表示为每100个有丝分裂细胞中异常有丝分裂细胞的数量。使用t检验将测试组（一式三份）的MI和GT与阴性（水）对照组进行比较。调制效果（ME）的计算公式为ME（％）=（1-（A-B）/（A-C）×100）-1。 ME阳性表示增加（协同作用或增强作用），ME阴性表示诱变剂引起的基因毒性降低（抗基因毒性）。 D. anomala提取物的浓度具有细胞毒性和遗传毒性。具有较低浓度D. anomala提取物的CP或EMS混合物的遗传毒性比单独使用CP或EMS（分别增加250.50％）或EMS（分别增加149.74和157.37％）的遗传毒性更大。仅有0.313 mg mL-1的相册隐孢子虫提取物具有细胞毒性，但三个浓度均无遗传毒性。 CP与C. album提取物的混合物具有细胞毒性。低浓度（50.72，61.13％）的贯叶连翘提取物可降低CP诱导的遗传毒性（55.18，68.36，57.40％），而EMS诱导的遗传毒性也可降低。但是，0.313 mg mL-1 C.相册提取物增加（236.75％）EMS诱导的遗传毒性。 C. sinensis提取物及其与CP的混合物无细胞毒性或遗传毒性。 C. sinensis提取物降低了CP诱导的遗传毒性（63.61，66.62，78.64％），但是EMS诱导的遗传毒性增加了（124.97，4.48，110.52％）。

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