...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Health: A Global Access Science Source >Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde–free laboratory is possible
【24h】

Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde–free laboratory is possible

机译：评估两种商业和三种自制固定剂以替代福尔马林：无甲醛实验室是可能的

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Formaldehyde (HCHO) is a gas (available as a 37% concentrated solution, stabilized with methanol). The 10% dilution (approximately 4% formaldehyde) has been used as a fixative since the end of the 19th century. Alternative fixatives are also commercially available or may be prepared in-house in laboratories. Statements by the IARC, along with other USA agencies (CalEPA, RoC/NTP) on the carcinogenicity of formaldehyde for humans renders its substitution in Pathology Departments necessary since the annual use of formalin may exceed 3,500 liters for a medium-large laboratory. To achieve a “formalin-free laboratory” we tested straightforward-to-make fixatives along with registered reagents offered as formalin substitutes. Methods More than two hundreds specimens were fixed in parallel with in-laboratory made fixatives PAGA (Polyethylenglycol, ethyl Alcohol, Glycerol, Acetic acid), two zinc-based fixatives (ZBF, Z7), and commercially-available alternatives (RCL2 and CellBlock). Tissue micro arrays were used for morphological and immunohistochemical comparison. Extraction of RNA was carried out to evaluate preservation of nucleic acids. Results Differences compared to formalin fixation were evident in alcohol-based fixatives, mainly restricted to higher stain affinity and considerable tissue shrinkage. Conversely, nuclear detail was superior with these alcohol-based formulas compared to formalin or glyoxale-based recipes. RNA extraction was superior for Z7, PAGA and RCL2 with regard to concentration but relatively comparable regarding quality. Conclusions Abolition of the human carcinogen formaldehyde from pathology laboratories is possible even in contexts whereby commercial alternatives to formalin are unavailable or are too expensive for routine use, and aspiration devices are lacking or not adequately serviced. The use of known formulations, possibly with simple and not-noxious (“alimentary grade”) constituents, comparable with registered proprietary products, may expand the search for the ideal fixative combining satisfactory morphology with improved preservation of nucleic acids and proteins as well as being easy and safe to dispose of.
机译：背景技术甲醛（HCHO）是一种气体（以37％的浓溶液形式提供，用甲醇稳定）。自19世纪末以来，已将10％的稀释液（约4％的甲醛）用作固定剂。替代固定剂也可商购获得或可以在实验室内部制备。 IARC以及其他美国机构（CalEPA，RoC / NTP）关于甲醛对人类致癌性的声明使得在病理学部门有必要替代甲醛，因为对于中型实验室而言，福尔马林的年使用量可能超过3500升。为了实现“无福尔马林实验室”，我们测试了易于制作的固定剂以及作为福尔马林替代品提供的注册试剂。方法用实验室制备的固定剂PAGA（聚乙烯乙二醇，乙醇，甘油，乙酸，乙酸），两种锌基固定剂（ZBF，Z7）和市售替代品（RCL2和CellBlock）平行固定两百多个标本。组织微阵列用于形态学和免疫组织化学比较。进行RNA的提取以评估核酸的保存。结果在基于酒精的固定剂中，与福尔马林固定相比存在明显差异，主要限于较高的染色亲和力和明显的组织收缩。相反，与基于福尔马林或乙二醛的配方相比，这些基于醇的配方的核细节更为优越。就浓度而言，RNA提取优于Z7，PAGA和RCL2，但质量相对可比。结论即使在无法获得福尔马林商业替代品或日常使用的商业替代品太昂贵，缺少或没有充分使用抽吸装置的情况下，也可以从病理学实验室废除人类致癌甲醛。与注册专利产品相比，使用可能具有简单且无毒（“营养级”）成分的已知制剂，可能会扩大对理想固定剂的研究，将令人满意的形态与改善的核酸和蛋白质保存性相结合。简单安全地处置。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号