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首页> 外文期刊>Epigenetics & Chromatin >HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes
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HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes

机译：核转移到非洲爪蟾卵母细胞后转录重编程需要依赖HIRA的H3.3沉积

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Background Nuclear reprogramming is potentially important as a route to cell replacement and drug discovery, but little is known about its mechanism. Nuclear transfer to eggs and oocytes attempts to identify the mechanism of this direct route towards reprogramming by natural components. Here we analyze how the reprogramming of nuclei transplanted to Xenopus oocytes exploits the incorporation of the histone variant H3.3. Results After nuclear transplantation, oocyte-derived H3.3 but not H3.2, is deposited on several regions of the genome including rDNA, major satellite repeats, and the regulatory regions of Oct4. This major H3.3 deposition occurs in absence of DNA replication, and is HIRA-and transcription-dependent. It is necessary for the shift from a somatic- to an oocyte-type of transcription after nuclear transfer. Conclusions This study demonstrates that the incorporation of histone H3.3 is an early and necessary step in the direct reprogramming of somatic cell nuclei by oocyte. It suggests that the incorporation of histone H3.3 is necessary during global changes in transcription that accompany changes in cell fate.
机译：背景技术核重编程作为细胞置换和药物发现的途径可能非常重要，但对其机制知之甚少。核转移到卵和卵母细胞试图确定这种直接途径对天然成分重新编程的机制。在这里，我们分析如何重编程移植到非洲爪蟾卵母细胞的核利用组蛋白变体H3.3的纳入。结果核移植后，卵母细胞衍生的H3.3而非H3.2沉积在基因组的多个区域，包括rDNA，主要卫星重复序列和Oct4的调控区域。主要的H3.3沉积发生在没有DNA复制的情况下，并且是HIRA和转录依赖性的。核转移后，从体细胞型转变为卵母细胞型转录是必要的。结论这项研究表明组蛋白H3.3的掺入是卵母细胞直接重编程体细胞核的早期和必要步骤。这表明在细胞命运变化伴随的转录整体变化过程中，组蛋白H3.3的掺入是必要的。

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